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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 42, Iss. 16 — Jun. 1, 2003
  • pp: 3005–3008

Absorption coefficient imaging by near-field scanning optical microscopy in bacteria

Ana M. de Paula, Claudilene R. Chaves, Haroldo B. Silva, and Gerald Weber  »View Author Affiliations


Applied Optics, Vol. 42, Issue 16, pp. 3005-3008 (2003)
http://dx.doi.org/10.1364/AO.42.003005


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Abstract

We present a method for obtaining a position-dependent absorption coefficient from near-field scanning optical transmission microscopy. We show that the optical transmission intensity can be combined with the topography, resulting into an absorption coefficient that simplifies the analysis of different materials within a sample. The method is tested with the dye rhodamine 6G, and we show some analysis in biological samples such as bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa. The calculated absorption coefficient images show important details of the bacteria, in particular for P. aeruginosa, in which membrane vesicles are clearly seen.

© 2003 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.5810) Microscopy : Scanning microscopy
(300.1030) Spectroscopy : Absorption

History
Original Manuscript: August 19, 2002
Revised Manuscript: February 5, 2003
Published: June 1, 2003

Citation
Ana M. de Paula, Claudilene R. Chaves, Haroldo B. Silva, and Gerald Weber, "Absorption coefficient imaging by near-field scanning optical microscopy in bacteria," Appl. Opt. 42, 3005-3008 (2003)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-42-16-3005


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References

  1. D. W. Pohl, W. Denk, M. Lanz, “Optical stethoscopy: image recording with resolution λ/20,” Appl. Phys. Lett. 44, 651–653 (1984). [CrossRef]
  2. U. Dürig, D. W. Pohl, F. Rohner, “Near-field optical-scanning microscopy,” J. Appl. Phys. 59, 3318–3327 (1986). [CrossRef]
  3. E. Betzig, J. K. Trautman, T. D. Harris, J. S. Weiner, R. L. Kostelak, “Breaking the diffraction barrier: optical microscopy on a nanometric scale,” Science 251, 1468–1470 (1991). [CrossRef] [PubMed]
  4. E. Betzig, P. L. Finn, J. S. Weiner, “Combined shear force and near-field scanning optical microscopy,” Appl. Phys. Lett. 60, 2484–2486 (1992). [CrossRef]
  5. T. Enderle, T. Ha, D. S. Chemla, S. Weiss, “Near-field fluorescence microscopy of cells,” Ultramicroscopy 71, 303–309 (1998). [CrossRef] [PubMed]
  6. A. Penzkofer, E. Drotleff, W. Holzer, “Optical constants measurement of single-layer thin films on transparent substrates,” Opt. Commun. 158, 221–230 (1998). [CrossRef]
  7. R. Carminati, A. Madrazo, M. Nieto-Vesperinas, J.-J. Greffet, “Optical content and resolution of near-field optical images: influence of the operating mode,” J. Appl. Phys. 82, 501–509 (1997). [CrossRef]
  8. G. W. Bryant, “Probing quantum nanostructures with near-field optical microscopy and vice versa,” Appl. Phys. Lett. 72, 768–770 (1998). [CrossRef]
  9. R. Stevenson, D. Richards, “The use of a near-field probe for the study of semiconductor heterostructures,” Semicond. Sci. Technol. 13, 882–886 (1998). [CrossRef]
  10. H. B. Silva, J. A. Toledo, K. Silveira Filho, G. Weber, A. M. de Paula, “Near-field scanning optical transmission images in Pseudomonas aeruginosa,” Acta Microscopica 10, 99–102 (2001).
  11. N. Ben-Ami, A. Radko, U. Ben-Ami, K. Lieberman, Z. Rothman, I. Rabin, A. Lewis, “Near-field optical imaging of unstained bacteria: comparison with normal atomic force and far-field optical microscopy in air and aqueous media,” Ultramicroscopy 71, 321–325 (1998). [CrossRef] [PubMed]
  12. J. L. Kadurugamuwa, T. J. Beveridge, “Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion,” J. Bacteriol. 177, 3998–4008 (1995). [PubMed]

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