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Applied Optics

Applied Optics


  • Vol. 42, Iss. 19 — Jul. 1, 2003
  • pp: 3927–3934

Multispectral laser-induced fluorescence imaging system for large biological samples

Moon S. Kim, Alan M. Lefcourt, and Yud-Ren Chen  »View Author Affiliations

Applied Optics, Vol. 42, Issue 19, pp. 3927-3934 (2003)

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A laser-induced fluorescence imaging system developed to capture multispectral fluorescence emission images simultaneously from a relatively large target object is described. With an expanded, 355-nm Nd:YAG laser as the excitation source, the system captures fluorescence emission images in the blue, green, red, and far-red regions of the spectrum centered at 450, 550, 678, and 730 nm, respectively, from a 30-cm-diameter target area in ambient light. Images of apples and of pork meat artificially contaminated with diluted animal feces have demonstrated the versatility of fluorescence imaging techniques for potential applications in food safety inspection. Regions of contamination, including sites that were not readily visible to the human eye, could easily be identified from the images.

© 2003 Optical Society of America

OCIS Codes
(110.4190) Imaging systems : Multiple imaging
(150.3040) Machine vision : Industrial inspection
(170.0110) Medical optics and biotechnology : Imaging systems
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

Original Manuscript: November 12, 2002
Revised Manuscript: March 13, 2003
Published: July 1, 2003

Moon S. Kim, Alan M. Lefcourt, and Yud-Ren Chen, "Multispectral laser-induced fluorescence imaging system for large biological samples," Appl. Opt. 42, 3927-3934 (2003)

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