OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 42, Iss. 19 — Jul. 1, 2003
  • pp: 3935–3943

Automated Detection of Fecal Contamination of Apples by Multispectral Laser-Induced Fluorescence Imaging

Alan M. Lefcourt, Moon S. Kim, and Yud-Ren Chen  »View Author Affiliations


Applied Optics, Vol. 42, Issue 19, pp. 3935-3943 (2003)
http://dx.doi.org/10.1364/AO.42.003935


View Full Text Article

Acrobat PDF (1248 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Animal feces are a suspected source of contamination of apples by disease-causing organisms such as Escherichia coli O157. Laser-induced fluorescence was used to detect different amounts of feces from dairy cows, deer, and a dairy pasture applied to Red Delicious apples. One day after application, detection for 1:2 and 1:20 dilutions was nearly 100%, and for 1:200 dilutions (<15 ng of dry matter) detection was >80%. Detection after apples had been washed and brushed was lowest for pasture feces; detection for 1:2, 1:20, and 1:200 dilutions of feces was 100%, 30%, and 0%, respectively. This technology may encourage development of commercial systems for detecting fecal contamination of apples.

© 2003 Optical Society of America

OCIS Codes
(150.3040) Machine vision : Industrial inspection
(170.0110) Medical optics and biotechnology : Imaging systems
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

Citation
Alan M. Lefcourt, Moon S. Kim, and Yud-Ren Chen, "Automated Detection of Fecal Contamination of Apples by Multispectral Laser-Induced Fluorescence Imaging," Appl. Opt. 42, 3935-3943 (2003)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-42-19-3935


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. G. L. Armstrong, J. Hollingsworth, and J. G. Morris, Jr., “Emerging foodborne pathogens: Escherichia coli O157: H7 as a model of entry of a new pathogen into the food supply of the developed world,” Epidemiol. Rev. 18, 29–51 (1996).
  2. R. L. Buchanan and M. P. Doyle, “Foodborne disease significance of Escherichia coli 0157:H7 and other enterohemorrhagic E. coli,” Food Technol. 51, 69–76 (1997).
  3. R. E. Brackett, “Incidence, contributing factors, and control of bacterial pathogens in produce,” Postharvest Biol. Technol. 15, 305–311 (1999).
  4. P. S. Mead, L. Slutsker, V. Dietz, L. F. McCaig, J. S. Bresee, C. Shapiro, P. M. Griffin, and R. V. Tauxe, “Food-related illness and death in the United States,” Emerg. Infect. Diseases 5, 607–625 (1999).
  5. B. T. Steele, N. Murphy, G. S. Arbus, and C. P. Rance, “An outbreak of hemolytic uremic syndrome associated with the ingestion of fresh apple juice,” J. Pediatr. 101, 963–965 (1982).
  6. R. E. Besser, S. M. Lett, J. T. Weber, M. P. Doyle, T. J. Barret, J. G. Wells, and P. M. Griffin, “An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli 0157:H7 in fresh pressed apple cider,” J. Am. Med. Assoc. 269, 2217–2220 (1993).
  7. Centers for Disease Control and Prevention, “Outbreak of E. coli 0157:H7 infections associated with drinking unpasteurized commercial apple juice—October 1996,” Morbid. Mortal. Weekly Rep. 45, 975–982 (1996).
  8. Centers for Disease Control and Prevention, “Outbreaks of Escherichia coli 0157:H7 infection and cryptosporidiosis associated drinking unpasteurized apple cider—Connecticut and New York, October 1996,” Morbid. Mortal. Weekly Rep. 46, 4–8 (1997).
  9. S. H. Cody, M. K. Glynn, J. A. Farrar, K. L. Cairns, P. M. Griffin, J. Kobayashi, M. Fyfe, R. Hoffman, A. S. King, J. H. Lewis, B. Swaminathan, R. G. Bryant, and D. J. Vugia, “An outbreak of Escherichia coli O157:H7 infection from unpasteurized commercial apple juice,” Ann. Internal Med. 130, 202–209 (1999).
  10. M. M. Lang, S. C. Ingham, and B. H. Ingham, “Verifying apple cider plant sanitation and hazard analysis crtical control point programs: choices for indicator bacteria and testing,” J. Food Protect. 62, 887–893 (1999).
  11. H. E. Uljas and S. C. Ingham, “Survey of apple growing, harvesting, and cider manufacturing practices in Wisconsin: implications for safety,” J. Food Safety 20, 85–100 (2000).
  12. D. C. R. Riordan, G. M. Sapers, T. R. Hankinson, M. Magee, A. M. Mattrazzo, and B. A. Annous, “A study of U. S. orchards to identify potential sources of Escherichia coli 0157:H7,” J. Food Protect. 64, 1320–1327 (2001).
  13. J. S. Wallace, T. Cheasty, and K. Jones, “Isolation of vero cytotoxin-producing Escherichia coli 0157 from wild birds,” J. Appl. Microbiol. 82, 399–404 (1997).
  14. Food Safety Inspection Service, U.S. Department of Agriculture, “Livestock post-mortem inspection activities-enforcing the zero tolerances for fecal materials, ingesta, and milk,” Directive 6420.1, http://www.fsis.usda.gov/oppde/rdad/fsisdirectives/fsisdir6420_1.htm (1998), pp. 1–9.
  15. U.S. Food and Drug Administration, “Hazard analysis and critical control point (HAACP); procedures for the safe and sanitary processing and importing of juices,” Fed. Regist. 66, 6137–6202 (2001).
  16. M. S. Kim, A. M. Lefcourt, Y. R. Chen, I. Kim, K. Chao, and D. Chan, “Multispectral detection of fecal contamination on apples based on hyperspectral imagery. II. Application of fluorescence imaging,” Trans. ASAE 45, 2027–2038 (2002).
  17. M. S. Kim, A. M. Lefcourt, K. Chao, Y. R. Chen, and I. Kim, “Multispectral detection of fecal contamination on apples based on hyperspectral imagery. I. Application of visible-near infrared reflectance imaging,” Trans. ASAE 45, 2017–2026 (2002).
  18. M. S. Kim, A. M. Lefcourt, and Y.-R. Chen, “Multispectral laser-induced fluorescence imaging system,” Appl. Opt. 42, 3927–3934 (2003).
  19. A. R. Weeks, Jr., Fundamentals of Electronic Image Processing (SPIE Press, Bellingham, Wash., 1996).
  20. M. S. Kim, A. M. Lefcourt, and Y. R. Chen, “Optimal fluorescence excitation and emission bands for detection of fecal contamination,” J. Food Prod. (to be published).
  21. Y. Tao and Z. Wen, “Adaptive spherical transforms for high-speed fruit defect detection,” Trans. ASAE 42, 241–246 (1998).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited