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Applied Optics

Applied Optics


  • Vol. 42, Iss. 19 — Jul. 1, 2003
  • pp: 3935–3943

Automated detection of fecal contamination of apples by multispectral laser-induced fluorescence imaging

Alan M. Lefcourt, Moon S. Kim, and Yud-Ren Chen  »View Author Affiliations

Applied Optics, Vol. 42, Issue 19, pp. 3935-3943 (2003)

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Animal feces are a suspected source of contamination of apples by disease-causing organisms such as Escherichia coli O157. Laser-induced fluorescence was used to detect different amounts of feces from dairy cows, deer, and a dairy pasture applied to Red Delicious apples. One day after application, detection for 1:2 and 1:20 dilutions was nearly 100%, and for 1:200 dilutions (<15 ng of dry matter) detection was >80%. Detection after apples had been washed and brushed was lowest for pasture feces; detection for 1:2, 1:20, and 1:200 dilutions of feces was 100%, 30%, and 0%, respectively. This technology may encourage development of commercial systems for detecting fecal contamination of apples.

© 2003 Optical Society of America

OCIS Codes
(150.3040) Machine vision : Industrial inspection
(170.0110) Medical optics and biotechnology : Imaging systems
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

Original Manuscript: November 12, 2002
Revised Manuscript: March 13, 2003
Published: July 1, 2003

Alan M. Lefcourt, Moon S. Kim, and Yud-Ren Chen, "Automated detection of fecal contamination of apples by multispectral laser-induced fluorescence imaging," Appl. Opt. 42, 3935-3943 (2003)

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