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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 42, Iss. 19 — Jul. 1, 2003
  • pp: 4119–4124

Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples

Takashi Fukano and Atsushi Miyawaki  »View Author Affiliations


Applied Optics, Vol. 42, Issue 19, pp. 4119-4124 (2003)
http://dx.doi.org/10.1364/AO.42.004119


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Abstract

We have developed a whole-field fluorescence microscope equipped with a Digital Micromirror Device to acquire optically sectioned images by using the fringe-projection technique and the phase-shift method. This system allows free control of optical sectioning strength through computer-controlled alteration of the fringe period projected onto a sample. We have employed this system to image viable cells expressing fluorescent proteins and discussed its biological applications.

© 2003 Optical Society of America

OCIS Codes
(100.2650) Image processing : Fringe analysis
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(180.2520) Microscopy : Fluorescence microscopy
(230.3990) Optical devices : Micro-optical devices
(230.6120) Optical devices : Spatial light modulators

History
Original Manuscript: December 2, 2002
Revised Manuscript: April 10, 2003
Published: July 1, 2003

Citation
Takashi Fukano and Atsushi Miyawaki, "Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples," Appl. Opt. 42, 4119-4124 (2003)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-42-19-4119

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