OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 42, Iss. 28 — Oct. 1, 2003
  • pp: 5693–5700

Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

Lisong Yang, Cian M. Taylor, Yury Rakovich, and Eithne M. McCabe  »View Author Affiliations


Applied Optics, Vol. 42, Issue 28, pp. 5693-5700 (2003)
http://dx.doi.org/10.1364/AO.42.005693


View Full Text Article

Enhanced HTML    Acrobat PDF (834 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We experimentally studied the three-dimensional imaging of the microspheres by using confocal and conventional scanning polarization microscopes. Because of the field amplitude averaging effect of the confocal system, the polarization of the detected signals is mainly parallel to the initial polarization. As a result, the signal intensity from the microspheres in the confocal polarization microscope with a crossed analyzer was found to be weaker than that in the conventional system. Based on a vector approach that takes the polarization into account and on the image formations of the two systems, theoretical expressions are given that agree well with the experimental results.

© 2003 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(260.5430) Physical optics : Polarization
(290.5850) Scattering : Scattering, particles

History
Original Manuscript: April 7, 2003
Revised Manuscript: April 14, 2003
Published: October 1, 2003

Citation
Lisong Yang, Cian M. Taylor, Yury Rakovich, and Eithne M. McCabe, "Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes," Appl. Opt. 42, 5693-5700 (2003)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-42-28-5693


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. A. F. Hallimond, The Polarizing Microscope (Vickers, York, UK, 1970).
  2. H. Kubota, S. Inoué, “Diffraction images in the polarizing microscope,” J. Opt. Soc. Am. 49, 191–198 (1959). [CrossRef] [PubMed]
  3. T. Wilson, R. Juškaitis, “On the extinction coefficient in confocal polarization microscopy,” J. Microsc. 179, 238–240 (1995). [CrossRef]
  4. T. Wilson, R. Juškaitis, P. Higdon, “The imaging of dielectric point scatterers in conventional and confocal polarisation microscopes,” Opt. Commun. 141, 298–313 (1997). [CrossRef]
  5. P. Török, P. D. Higdon, T. Wilson, “Theory for confocal and conventional microscopes imaging small dielectric scatterers,” J. Mod. Opt. 45, 1681–1698 (1998). [CrossRef]
  6. P. Török, P. D. Higdon, T. Wilson, “On the general properties of polarised light conventional and confocal microscopes,” Opt. Commun. 148, 300–315 (1998). [CrossRef]
  7. V. Sandoghdar, F. Treussart, J. Hare, V. Lefevre-Seguin, J. M. Raimond, S. Haroche, “A very low threshold whispering-gallery mode microsphere laser,” Phys. Rev. A 54, R1777–R1780 (1996). [CrossRef]
  8. S. M. Spillane, T. J. Kippenberg, K. J. Vahala, “Ultralow-threshold Raman laser using a spherical dielectric microcavity,” Nature 415, 621–623 (2002). [CrossRef] [PubMed]
  9. A. Ashkin, “Optical trapping and manipulation of neutral particles using lasers,” Proc. Natl. Acad. Sci. USA 94, 4853–4860 (1997). [CrossRef] [PubMed]
  10. A. Ashkin, J. M. Dziedzic, “Observation of resonances in the radiation pressure on dielectric spheres,” Phys. Rev. Lett. 38, 1351–1354 (1977). [CrossRef]
  11. M. Fayngold, “Spherical particle imaging and glare-ring spectroscopy with backscattered light,” J. Opt. Soc. Am. A 14, 1825–1835 (1997). [CrossRef]
  12. W. Inami, Y. Kawata, “Three-dimensional imaging analysis of confocal and conventional polarization microscopes by use of Mie scattering theory,” Appl. Opt. 39, 6369–6373 (2000). [CrossRef]
  13. J. P. Barton, D. R. Alexander, S. A. Schaub, “Internal and near-surface electromagnetic fields for a spherical particle irradiated by a focused laser beam,” J. Appl. Phys. 64, 1632–1639 (1988). [CrossRef]
  14. T. Wilson, Confocal Microscopy (Academic, London, 1990).
  15. J. Mahler, G. Rafler, “Modified melamine resins for optical applications,” Opt. Mater. 12, 363–368 (1999). [CrossRef]
  16. W. T. Grandy, Scattering of Waves from Large Spheres (Cambridge U. Press, Cambridge, UK, 2000). [CrossRef]
  17. A. Ashkin, J. M. Dziedzic, “Observation of optical resonances of dielectric spheres by light scattering,” Appl. Opt. 20, 1803–1814 (1981). [CrossRef] [PubMed]
  18. I. L. Radtchenko, G. B. Sukhorukov, N. Gaponik, A. Kornowski, A. L. Rogach, H. Möhwald, “Core-shell structures formed by the solvent-controlled precipitation of luminescent CdTe nanocrystals on latex spheres,” Adv. Mater. 13, 1684–1687 (2001). [CrossRef]
  19. A. S. Susha, F. Caruso, A. L. Rogach, G. B. Sukhorukov, A. Kornowski, H. Möhwald, M. Giersig, A. Eychmuller, H. Weller, “Formation of luminescent spherical core-shell particles by the consecutive adsorption of polyelectrolyte and CdTe(S) nanocrystals on latex colloids,” Colloids Surf. A 163, 39–44 (2000). [CrossRef]
  20. B. Möller, M. V. Artemyev, U. Woggon, R. Wannemacher, “Mode identification in spherical microcavities doped with quantum dots,” Appl. Phys. Lett. 80, 3253–3255 (2002). [CrossRef]
  21. Y. Rakovich, J. F. Donegan, N. P. Gaponik, A. L. Rogach, “Whispering gallery mode emission from a core-shell system of CdTe nanocrystals on a spherical microcavity,” in Physics, Chemistry and Application of Nanostructures, V. E. Borisenko, S. V. Gaponenko, V. S. Gurin, eds. (World Scientific, Singapore, 2003), pp. 120–123.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited