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Applied Optics

Applied Optics


  • Vol. 42, Iss. 28 — Oct. 1, 2003
  • pp: 5693–5700

Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

Lisong Yang, Cian M. Taylor, Yury Rakovich, and Eithne M. McCabe  »View Author Affiliations

Applied Optics, Vol. 42, Issue 28, pp. 5693-5700 (2003)

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We experimentally studied the three-dimensional imaging of the microspheres by using confocal and conventional scanning polarization microscopes. Because of the field amplitude averaging effect of the confocal system, the polarization of the detected signals is mainly parallel to the initial polarization. As a result, the signal intensity from the microspheres in the confocal polarization microscope with a crossed analyzer was found to be weaker than that in the conventional system. Based on a vector approach that takes the polarization into account and on the image formations of the two systems, theoretical expressions are given that agree well with the experimental results.

© 2003 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(260.5430) Physical optics : Polarization
(290.5850) Scattering : Scattering, particles

Original Manuscript: April 7, 2003
Revised Manuscript: April 14, 2003
Published: October 1, 2003

Lisong Yang, Cian M. Taylor, Yury Rakovich, and Eithne M. McCabe, "Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes," Appl. Opt. 42, 5693-5700 (2003)

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