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Applied Optics

Applied Optics


  • Vol. 42, Iss. 28 — Oct. 1, 2003
  • pp: 5737–5743

Diattenuation and polarization preservation of retinal nerve fiber layer reflectance

Xiang-Run Huang and Robert W. Knighton  »View Author Affiliations

Applied Optics, Vol. 42, Issue 28, pp. 5737-5743 (2003)

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The diattenuation spectrum of the retinal nerve fiber layer (RNFL) reflectance has been predicted to depend strongly on the relative refractive index (m) of light-scattering cylinders. To constrain the values of m, diattenuation of the RNFL reflectance of isolated rat retina was measured with a multispectral imaging micropolarimeter. The RNFL reflection has very weak intrinsic diattenuation at all wavelengths (400–830 nm), which rejects all values of m ≥ 1.03. Degree of polarization (DOP) for reflection from the RNFL was also measured. DOP was close to unity at all wavelengths, which indicates that the RNFL is a polarization-preserving reflector.

© 2003 Optical Society of America

OCIS Codes
(260.2130) Physical optics : Ellipsometry and polarimetry
(260.5430) Physical optics : Polarization
(290.5850) Scattering : Scattering, particles

Original Manuscript: January 8, 2003
Revised Manuscript: June 4, 2003
Published: October 1, 2003

Xiang-Run Huang and Robert W. Knighton, "Diattenuation and polarization preservation of retinal nerve fiber layer reflectance," Appl. Opt. 42, 5737-5743 (2003)

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  1. S. C. Pollock, N. R. Miller, “The retinal nerve fiber layer,” Int. Ophthalmol. Clin. 26, 201–221 (1986). [CrossRef] [PubMed]
  2. A. W. Dreher, K. Reiter, “Retinal laser ellipsometry—a new method for measuring the retinal nerve-fiber layer thickness distribution,” Clin. Vis. Sci. 7, 481–488 (1992).
  3. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991). [CrossRef] [PubMed]
  4. J. S. Schuman, M. R. Hee, C. A. Puliafito, C. Wong, T. Pedut-Kloizman, C. P. Lin, E. Hertzmark, J. A. Izatt, E. A. Swanson, J. G. Fujimoto, “Quantification of nerve fiber layer thickness in normal and glaucomatous eyes using optical coherence tomography,” Arch. Ophthalmol. 113, 586–596 (1995). [CrossRef] [PubMed]
  5. R. O. Burk, H. E. Volcker, “Current imaging of the optic disk and retinal nerve fiber layer,” Curr. Opin. Ophthalmol. 7, 99–108 (1996). [CrossRef] [PubMed]
  6. D. S. Greenfield, “Optic nerve and retinal nerve fiber layer analyzers in glaucoma,” Curr. Opin. Ophthalmol. 13, 68–76 (2002). [CrossRef] [PubMed]
  7. R. N. Weinreb, A. W. Dreher, A. Coleman, H. Quigley, B. Shaw, K. Reiter, “Histopathologic validation of Fourier-ellipsometry measurements of retinal nerve fiber layer thickness,” Arch. Ophthalmol. 108, 557–560 (1990). [CrossRef] [PubMed]
  8. R. W. Knighton, Q. Zhou, “The relation between reflectance and thickness of the retinal nerve fiber layer,” J. Glaucoma 4, 117–123 (1995). [CrossRef] [PubMed]
  9. R. W. Knighton, X.-R. Huang, “Directional and spectral reflectance of the rat retinal nerve fiber layer,” Invest. Ophthalmol. Vis. Sci. 40, 639–647 (1999). [PubMed]
  10. B. Cense, T. C. Chen, B. H. Park, M. C. Pierce, J. F. de Boer, “In vivo depth-resolved birefringence measurements of the human retinal nerve fiber layer by polarization-sensitive optical coherence tomography,” Opt. Lett. 27, 1610–1612 (2002). [CrossRef]
  11. Q. Zhou, R. W. Knighton, “Light scattering and form birefringence of parallel cylindrical arrays that represent cellular organelles of the retinal nerve fiber layer,” Appl. Opt. 36, 2273–2285 (1997). [CrossRef] [PubMed]
  12. X.-R. Huang, R. W. Knighton, “Theoretical model of the polarization properties of the retinal nerve fiber layer in reflection,” Appl. Opt. 42, 5726–5736 (2003). [CrossRef] [PubMed]
  13. R. A. Chipman, “Polarimetry,” in Handbook of Optics, 2nd ed., M. Bass, E. W. van Stryland, D. R. Williams, W. L. Wolfe, eds. (McGraw-Hill, New York, 1995), pp. 22.21–22.37.
  14. G. J. van Blokland, “Ellipsometry of the human retina in vivo: preservation of polarization,” J. Opt. Soc. Am. A 2, 72–75 (1985). [CrossRef] [PubMed]
  15. A. W. Dreher, K. Reiter, R. N. Weinreb, “Spatially resolved birefringence of the retinal nerve-fiber layer assessed with a retinal laser ellipsometer,” Appl. Opt. 31, 3730–3735 (1992). [CrossRef] [PubMed]
  16. X.-R. Huang, R. W. Knighton, “Linear birefringence of the retinal nerve fiber layer measured in vitro with a multispectral imaging micropolarimeter,” J. Biomed. Opt. 7, 199–204 (2002). [CrossRef] [PubMed]
  17. X.-R. Huang, “Polarization properties of the retinal nerve fiber layer investigated with multispectral imaging micropolarimetry,” Ph.D. dissertation (University of Miami, Coral Gables, Fla., 2000).
  18. P. S. Hauge, “Mueller matrix ellipsometry with imperfect compensators,” J. Opt. Soc. Am. 68, 1519–1528 (1978). [CrossRef]
  19. R. W. Knighton, X.-R. Huang, “Visible and near-infrared imaging of the nerve fiber layer of the isolated rat retina,” J. Glaucoma 8, 31–37 (1999). [CrossRef] [PubMed]
  20. R. W. Knighton, C. Baverez, A. Bhattacharya, “The directional reflectance of the retinal nerve fiber layer of the toad,” Invest. Ophthalmol. Vis. Sci. 33, 2603–2611 (1992). [PubMed]
  21. D. S. Kliger, J. W. Lewis, C. E. Randall, Polarized Light in Optics and Spectroscopy (Academic, New York, 1990).
  22. C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles (Wiley, New York, 1983).
  23. H. C. van de Hulst, Light Scattering by Small Particles (Dover, New York, 1957).
  24. E. Collett, Polarized Light: Fundamentals and Applications (Marcel Dekker, New York, 1993).
  25. R. M. A. Azzam, N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, New York, 1989).
  26. A. M. Benoit, K. Naoun, V. Louis-Dorr, L. Mala, A. Raspiller, “Linear dichroism of the retinal nerve fiber layer expressed with Mueller matrices,” Appl. Opt. 40, 565–569 (2001). [CrossRef]
  27. R. W. Knighton, X.-R. Huang, Q. Zhou, “Microtubule contribution to the reflectance of the retinal nerve fiber layer,” Invest. Ophthalmol. Vis. Sci. 39, 189–193 (1998). [PubMed]
  28. H. Sato, G. W. Ellis, S. Inoue, “Microtubular origin of mitotic spindle form birefringence,” J. Cell Biol. 67, 501–517 (1975). [CrossRef] [PubMed]
  29. D. R. Williams, “Visual consequences of the foveal pit,” Invest. Ophthalmol. Vis. Sci. 19, 653–667 (1980). [PubMed]
  30. T. E. Ogden, “Nerve fiber layer astrocytes of the primate retina: morphology, distribution, and density,” Invest. Ophthalmol. Vis. Sci. 17, 499–510 (1978). [PubMed]

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