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Applied Optics

Applied Optics


  • Vol. 42, Iss. 30 — Oct. 20, 2003
  • pp: 6119–6132

Single-Pollen Analysis by Laser-Induced Breakdown Spectroscopy and Raman Microscopy

Ana R. Boyain-Goitia, David C. S. Beddows, Ben C. Griffiths, and Helmut H. Telle  »View Author Affiliations

Applied Optics, Vol. 42, Issue 30, pp. 6119-6132 (2003)

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The application of laser-induced breakdown spectroscopy to the analysis of single biological microparticles (bioaerosols) is described, exemplified here for a range of pollens. Spectra were recorded by exposure of the pollen to a single laser pulse from a Nd:YAG laser (λ = 1064 nm, Ep ~ 30 mJ). The intensities of the single-pulse laser-induced breakdown spectra fluctuated dramatically, but an internal signal calibration procedure was applied that referenced elemental line intensities to the carbon matrix of the sample (represented by molecular bands of CN and C2). This procedure allowed us to determine relative element concentration distributions for the different types of pollen. These pollens exhibited some distinct concentration variations, for both major and minor (trace) elements in the biomatrix, through which ultimately individual pollens might be identified and classified. The same pollen samples were also analyzed by Raman microscopy, which provided molecular compositional data (even with spatial resolution). These data allowed us to distinguish between biological and nonbiological specimens and to obtain additional classification information for the various pollen families, complementing the laser-induced breakdown spectroscopy measurement data.

© 2003 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(300.6360) Spectroscopy : Spectroscopy, laser
(300.6450) Spectroscopy : Spectroscopy, Raman

Ana R. Boyain-Goitia, David C. S. Beddows, Ben C. Griffiths, and Helmut H. Telle, "Single-Pollen Analysis by Laser-Induced Breakdown Spectroscopy and Raman Microscopy," Appl. Opt. 42, 6119-6132 (2003)

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