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Applied Optics

Applied Optics


  • Vol. 42, Iss. 30 — Oct. 20, 2003
  • pp: 6184–6191

Detection of bacteria by time-resolved laser-induced breakdown spectroscopy

Stéphane Morel, Nicolas Leone, Philippe Adam, and Jacques Amouroux  »View Author Affiliations

Applied Optics, Vol. 42, Issue 30, pp. 6184-6191 (2003)

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A laser-induced breakdown spectroscopy technique for analyzing biological matter for the detection of biological hazards is investigated. Eight species were considered in our experiment: six bacteria and two pollens in pellet form. The experimental setup is described, then a cumulative intensity ratio is proposed as a quantitative criterion because of its linearity and reproducibility. Time-resolved laser-induced breakdown spectroscopy (TRELIBS) exhibits a good ability to differentiate among all these species, whatever the culture medium, the species or the strain. Thus we expect that TRELIBS will be a good candidate for a sensor of hazards either on surfaces or in ambient air.

© 2003 Optical Society of America

OCIS Codes
(120.1880) Instrumentation, measurement, and metrology : Detection
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(140.3440) Lasers and laser optics : Laser-induced breakdown
(160.4890) Materials : Organic materials
(350.5400) Other areas of optics : Plasmas

Original Manuscript: January 21, 2003
Revised Manuscript: May 28, 2003
Published: October 20, 2003

Stéphane Morel, Nicolas Leone, Philippe Adam, and Jacques Amouroux, "Detection of bacteria by time-resolved laser-induced breakdown spectroscopy," Appl. Opt. 42, 6184-6191 (2003)

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