Laser-induced breakdown spectroscopy (LIBS) has been used to study bacterial spores, molds, pollens, and proteins. Biosamples were prepared and deposited onto porous silver substrates. LIBS data from the individual laser shots were analyzed by principal-components analysis and were found to contain adequate information to afford discrimination among the different biomaterials. Additional discrimination within the three bacilli studied appears feasible.

© 2003 Optical Society of America

[Optical Society of America ]

1. L. J. Radziemski, D. A. Cremers, and T. R. Loree, “Detection of beryllium by laser-induced breakdown spectroscopy,” Spectrochim. Acta Part B 38, 349–355 (1983).
2. K. Y. Yamamoto, D. A. Cremers, M. J. Ferris, and L. E. Foster, “Detection of metals in the environment using a portable laser-induced breakdown spectroscopy instrument,” Applied Spectrosc. 50, 222–233 (1996).
3. R. T. Wainner, R. S. Harmon, A. W. Miziolek, K. L. McNesby, and P. D. French, “Analysis of environmental lead contamination: comparison of LIBS field and laboratory instruments,” Spectrochim. Acta Part B 56, 777–793 (2001).
4. T. M. Moskal and D. W. Hahn, “On-line sorting of wood treated with chromated copper arsenate laser-induced breakdown spectroscopy,” Applied Spectrosc. 56, 1337–1344 (2002).
5. O. Samek, M. Liska, J. Kaiser, D. C. S. Beddows, H. H. Telle, and S. V. Kukhlevsky, “Clinical application of laser-induced breakdown spectroscopy to the analysis of teeth and dental materials,” J. Clin. Laser Med. Surg. 18, 281–289 (2000).
6. O. Samek, D. C. S. Beddows, H. H. Telle, G. W. Morris, M. Liska, and J. Kaiser, “Quantitative analysis of trace metal accumulation in teeth using laser-induced breakdown spectroscopy,” Appl. Phys. A 69, S179–S182 (1999).
7. A. R. Boyain-Goitia, D. C. S. Beddows, B. C. Griffiths, and H. H. Telle, “Single-pollen analysis by laser-induced breakdown spectroscopy and Raman microscopy,” Appl. Opt. 42, 6119–6132 (2003).
8. S. Morel, N. Leone, P. Adam, and J. Amouroux, “Detection of bacteria by time-resolved laser-induced breakdown spectroscopy,” Appl. Opt. 42, 6184–6191 (2003).
9. B. M. Wise, N. B. Gallagher, S. W. Butler, D. White, and G. G. Barna, “A comparison of principal components analysis, multi-way principal components analysis, tri-linear decomposition and parallel factor analysis for fault detection in a semiconductor etch process,” J. Chemometrics 13, 379–396 (1999).
10. K. R. Beebe, R. J. Pell, and M. B. Seasholtz, Chemometrics: A Practical Guide (Wiley-Interscience, New York, 1998).
11. M. Meloun, J. Capek, P. Miksik, and R. G. Brereton, “Critical comparison of methods predicting the number of components in spectroscopic data,” Anal. Chim. Acta 423, 51–68 (2000).
12. L. St-Onge, R. Sing, S. Bechard, and M. Sabsabi, “Carbon emissions following 1.064 μm laser ablation of graphite and organic sample in ambient air,” Appl. Phys. A 69, S913–S916 (1999).
13. J. Hybl, MIT Lincoln Laboratory, 244 Wood Street, Lexington, Mass. 02420 (personal communication, 2003).
14. B. Smith, Department of Chemistry, University of Florida, Gainesville, Fla. 32611 (personal communication, 2003).

Anal. Chim. Acta

• M. Meloun, J. Capek, P. Miksik, and R. G. Brereton, “Critical comparison of methods predicting the number of components in spectroscopic data,” Anal. Chim. Acta 423, 51–68 (2000).

Appl. Opt.

• A. R. Boyain-Goitia, D. C. S. Beddows, B. C. Griffiths, and H. H. Telle, “Single-pollen analysis by laser-induced breakdown spectroscopy and Raman microscopy,” Appl. Opt. 42, 6119–6132 (2003).
• S. Morel, N. Leone, P. Adam, and J. Amouroux, “Detection of bacteria by time-resolved laser-induced breakdown spectroscopy,” Appl. Opt. 42, 6184–6191 (2003).

Appl. Phys. A

• L. St-Onge, R. Sing, S. Bechard, and M. Sabsabi, “Carbon emissions following 1.064 μm laser ablation of graphite and organic sample in ambient air,” Appl. Phys. A 69, S913–S916 (1999).
• O. Samek, D. C. S. Beddows, H. H. Telle, G. W. Morris, M. Liska, and J. Kaiser, “Quantitative analysis of trace metal accumulation in teeth using laser-induced breakdown spectroscopy,” Appl. Phys. A 69, S179–S182 (1999).

Applied Spectrosc.

• K. Y. Yamamoto, D. A. Cremers, M. J. Ferris, and L. E. Foster, “Detection of metals in the environment using a portable laser-induced breakdown spectroscopy instrument,” Applied Spectrosc. 50, 222–233 (1996).
• T. M. Moskal and D. W. Hahn, “On-line sorting of wood treated with chromated copper arsenate laser-induced breakdown spectroscopy,” Applied Spectrosc. 56, 1337–1344 (2002).

J. Chemometrics

• B. M. Wise, N. B. Gallagher, S. W. Butler, D. White, and G. G. Barna, “A comparison of principal components analysis, multi-way principal components analysis, tri-linear decomposition and parallel factor analysis for fault detection in a semiconductor etch process,” J. Chemometrics 13, 379–396 (1999).

J. Clin. Laser Med. Surg.

• O. Samek, M. Liska, J. Kaiser, D. C. S. Beddows, H. H. Telle, and S. V. Kukhlevsky, “Clinical application of laser-induced breakdown spectroscopy to the analysis of teeth and dental materials,” J. Clin. Laser Med. Surg. 18, 281–289 (2000).

Spectrochim. Acta Part B

• R. T. Wainner, R. S. Harmon, A. W. Miziolek, K. L. McNesby, and P. D. French, “Analysis of environmental lead contamination: comparison of LIBS field and laboratory instruments,” Spectrochim. Acta Part B 56, 777–793 (2001).
• L. J. Radziemski, D. A. Cremers, and T. R. Loree, “Detection of beryllium by laser-induced breakdown spectroscopy,” Spectrochim. Acta Part B 38, 349–355 (1983).

Other

• J. Hybl, MIT Lincoln Laboratory, 244 Wood Street, Lexington, Mass. 02420 (personal communication, 2003).
• B. Smith, Department of Chemistry, University of Florida, Gainesville, Fla. 32611 (personal communication, 2003).
• K. R. Beebe, R. J. Pell, and M. B. Seasholtz, Chemometrics: A Practical Guide (Wiley-Interscience, New York, 1998).

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