OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 42, Iss. 30 — Oct. 20, 2003
  • pp: 6205–6209

Laser-induced breakdown spectroscopy of bacterial spores, molds, pollens, and protein: initial studies of discrimination potential

Alan C. Samuels, Frank C. DeLucia, Jr., Kevin L. McNesby, and Andrzej W. Miziolek  »View Author Affiliations

Applied Optics, Vol. 42, Issue 30, pp. 6205-6209 (2003)

View Full Text Article

Enhanced HTML    Acrobat PDF (94 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Laser-induced breakdown spectroscopy (LIBS) has been used to study bacterial spores, molds, pollens, and proteins. Biosamples were prepared and deposited onto porous silver substrates. LIBS data from the individual laser shots were analyzed by principal-components analysis and were found to contain adequate information to afford discrimination among the different biomaterials. Additional discrimination within the three bacilli studied appears feasible.

© 2003 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(300.6210) Spectroscopy : Spectroscopy, atomic
(300.6360) Spectroscopy : Spectroscopy, laser

Original Manuscript: March 5, 2003
Revised Manuscript: July 2, 2003
Published: October 20, 2003

Alan C. Samuels, Frank C. DeLucia, Kevin L. McNesby, and Andrzej W. Miziolek, "Laser-induced breakdown spectroscopy of bacterial spores, molds, pollens, and protein: initial studies of discrimination potential," Appl. Opt. 42, 6205-6209 (2003)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. L. J. Radziemski, D. A. Cremers, T. R. Loree, “Detection of beryllium by laser-induced breakdown spectroscopy,” Spectrochim. Acta Part B 38, 349–355 (1983). [CrossRef]
  2. K. Y. Yamamoto, D. A. Cremers, M. J. Ferris, L. E. Foster, “Detection of metals in the environment using a portable laser-induced breakdown spectroscopy instrument,” Applied Spectrosc. 50, 222–233 (1996). [CrossRef]
  3. R. T. Wainner, R. S. Harmon, A. W. Miziolek, K. L. McNesby, P. D. French, “Analysis of environmental lead contamination: comparison of LIBS field and laboratory instruments,” Spectrochim. Acta Part B 56, 777–793 (2001). [CrossRef]
  4. T. M. Moskal, D. W. Hahn, “On-line sorting of wood treated with chromated copper arsenate laser-induced breakdown spectroscopy,” Applied Spectrosc. 56, 1337–1344 (2002). [CrossRef]
  5. O. Samek, M. Liska, J. Kaiser, D. C. S. Beddows, H. H. Telle, S. V. Kukhlevsky, “Clinical application of laser-induced breakdown spectroscopy to the analysis of teeth and dental materials,” J. Clin. Laser Med. Surg. 18, 281–289 (2000).
  6. O. Samek, D. C. S. Beddows, H. H. Telle, G. W. Morris, M. Liska, J. Kaiser, “Quantitative analysis of trace metal accumulation in teeth using laser-induced breakdown spectroscopy,” Appl. Phys. A 69, S179–S182 (1999).
  7. A. R. Boyain-Goitia, D. C. S. Beddows, B. C. Griffiths, H. H. Telle, “Single-pollen analysis by laser-induced breakdown spectroscopy and Raman microscopy,” Appl. Opt. 42, 6119–6132 (2003). [CrossRef] [PubMed]
  8. S. Morel, N. Leone, P. Adam, J. Amouroux, “Detection of bacteria by time-resolved laser-induced breakdown spectroscopy,” Appl. Opt. 42, 6184–6191 (2003). [CrossRef] [PubMed]
  9. B. M. Wise, N. B. Gallagher, S. W. Butler, D. White, G. G. Barna, “A comparison of principal components analysis, multi-way principal components analysis, tri-linear decomposition and parallel factor analysis for fault detection in a semiconductor etch process,” J. Chemometrics 13, 379–396 (1999). [CrossRef]
  10. K. R. Beebe, R. J. Pell, M. B. Seasholtz, Chemometrics: A Practical Guide (Wiley-Interscience, New York, 1998).
  11. M. Meloun, J. Capek, P. Miksik, R. G. Brereton, “Critical comparison of methods predicting the number of components in spectroscopic data,” Anal. Chim. Acta 423, 51–68 (2000). [CrossRef]
  12. L. St-Onge, R. Sing, S. Bechard, M. Sabsabi, “Carbon emissions following 1.064 µm laser ablation of graphite and organic sample in ambient air,” Appl. Phys. A 69, S913–S916 (1999).
  13. J. Hybl, MIT Lincoln Laboratory, 244 Wood Street, Lexington, Mass. 02420 (personal communication, 2003).
  14. B. Smith, Department of Chemistry University of Florida Gainesville, Fla. 32611 (personal communication, 2003).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited