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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 10 — Apr. 1, 2004
  • pp: 2079–2088

Design, construction, characterization, and application of a hyperspectral microarray scanner

Michael B. Sinclair, Jerilyn A. Timlin, David M. Haaland, and Margaret Werner-Washburne  »View Author Affiliations


Applied Optics, Vol. 43, Issue 10, pp. 2079-2088 (2004)
http://dx.doi.org/10.1364/AO.43.002079


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Abstract

We describe the design, construction, and operation of a hyperspectral microarray scanner for functional genomic research. The hyperspectral instrument operates with spatial resolutions ranging from 3 to 30 μm and records the emission spectrum between 490 and 900 nm with a spectral resolution of 3 nm for each pixel of the microarray. This spectral information, when coupled with multivariate data analysis techniques, allows for identification and elimination of unwanted artifacts and greatly improves the accuracy of microarray experiments. Microarray results presented in this study clearly demonstrate the separation of fluorescent label emission from the spectrally overlapping emission due to the underlying glass substrate. We also demonstrate separation of the emission due to green fluorescent protein expressed by yeast cells from the spectrally overlapping autofluorescence of the yeast cells and the growth media.

© 2004 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

History
Original Manuscript: August 27, 2003
Revised Manuscript: January 5, 2004
Published: April 1, 2004

Citation
Michael B. Sinclair, Jerilyn A. Timlin, David M. Haaland, and Margaret Werner-Washburne, "Design, construction, characterization, and application of a hyperspectral microarray scanner," Appl. Opt. 43, 2079-2088 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-10-2079

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