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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 14 — May. 10, 2004
  • pp: 2861–2867

Determination of Collagen Fiber Orientation in Human Tissue by Use of Polarization Measurement of Molecular Second-Harmonic-Generation Light

Takeshi Yasui, Yoshiyuki Tohno, and Tsutomu Araki  »View Author Affiliations


Applied Optics, Vol. 43, Issue 14, pp. 2861-2867 (2004)
http://dx.doi.org/10.1364/AO.43.002861


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Abstract

Based on the reflection-type polarization measurement of second-harmonic-generation (SHG) light induced by collagen molecules, we are able to determine the collagen fiber orientation in human tissues taken from a cadaver. The resulting SHG radar graph shows the direction of the absolute orientation and the degree of organization of collagen fibers. To evaluate the probing sensitivity to the collagen orientation, we compared the proposed method with other polarimetric methods. Use of the proposed method revealed characteristic orientation differences among collagen fibers and demonstrated significant inhomogeneity with respect to the distribution of collagen orientation in human dentin. The proposed method provides a powerful research and diagnostic tool for examining the collagen orientation in human tissues.

© 2004 Optical Society of America

OCIS Codes
(120.5410) Instrumentation, measurement, and metrology : Polarimetry
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

Citation
Takeshi Yasui, Yoshiyuki Tohno, and Tsutomu Araki, "Determination of Collagen Fiber Orientation in Human Tissue by Use of Polarization Measurement of Molecular Second-Harmonic-Generation Light," Appl. Opt. 43, 2861-2867 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-14-2861


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References

  1. B. Eyden and M. Tzaphlidou, “Structural variations of collagen in normal and pathological tissues: role of electron microscopy,” Micron 32, 287–300 (2001).
  2. V. J. James, L. Delbridge, S. V. McLennan, and D. K. Yue, “Use of x-ray diffraction in study of human diabetic and aging collagen,” Diabetes 40, 391–394 (1991).
  3. S. Osaki, “Distribution map of collagen fiber orientation in a whole calf leather,” Anat. Rec. 254, 147–152 (1999).
  4. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254, 1178–1181 (1991).
  5. J. M. Schmitt, M. Yadlowsky, and R. F. Bonner, “Subsurface imaging of living skin with optical coherence microscopy,” Dermatology 191, 93 (1995).
  6. A. M. Rollins, R. Ung-Arunyawee, A. Chak, R. C. K. Wong, K. Kobayashi, M. V. Sivak, and J. A. Izatt, “Real-time in vivo imaging of human gastrointestinal ultrastructure by use of endoscopic optical coherence tomography with a novel efficient interferometer design,” Opt. Lett. 24, 1358–1360 (1999).
  7. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990).
  8. S. Fine and W. P. Hansen, “Optical second harmonic generation in biological tissues,” Appl. Opt. 10, 2350–2353 (1971).
  9. A. Zumbusch, G. R. Holtom, and X. S. Xie, “Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering,” Phys. Rev. Lett. 82, 4142–4145 (1999).
  10. S. Roth and I. Freund, “Optical second-harmonic scattering in rat-tail tendon,” Biopolymers 20, 1271–1290 (1981).
  11. B. M. Kim, J. Eichler, and L. B. Da Silva, “Frequency doubling of ultrashort laser pulses in biological tissues,” Appl. Opt. 38, 7145–7150 (1999).
  12. E. Georgiou, T. Theodossiou, V. Hovhannisyan, K. Politopoulos, G. S. Rapti, and D. Yova, “Second and third harmonic generation in type I collagen, by nanosecond laser radiation, over a broad spectral region,” Opt. Commun. 176, 253–260 (2000).
  13. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, “Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues,” Biophys. J. 82, 493–508 (2002).
  14. P. Stroller, K. M. Reiser, P. M. Celliers, and A. M. Rubenchik, “Polarization-modulated second harmonic generation in collagen,” Biophys. J. 82, 3330–3342 (2002).
  15. T. Yasui, K. Nakamoto, Y. Tohno, and T. Araki, “Laser induced dynamic emission sensitive to the conformation of tissue collagen,” in Proceeding of Switzerland-Japan Workshop on New Directions in Cellular and Tissues Biomechanics, J. J. Meister and K. Hayashi eds. (Switzerland-Japan Workshop, Switzerland, 2001), p. 85.
  16. T. Yasui, K. Shimabayashi, H. Kawaguchi, Y. Tohno, T. Iwata, and T. Araki, “Polarimetry of second-harmonic-generation light sensitive to orientation of tissue collagen,” in Abstract of International Conference on Optics Within Life Science VII, T. Tschudi, ed. (International Society on Optics Within Life Sciences, Switzerland, 2002), p. 19.
  17. P. Stroller, B.-M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. Da Silva, “Polarization-dependent optical second-harmonic imaging of a rat-tail tendon,” J. Biomed. Opt. 7, 205–214 (2002).
  18. A. T. Yeh, N. Nassif, A. Zoumi, and B. J. Tromberg, “Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence,” Opt. Lett. 27, 2082–2084 (2002).
  19. T. Yasui, Y. Tohno, and T. Araki, “Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry,” J. Biomed. Opt. 9, 259–264 (2004).
  20. D. J. Maitland, “Dynamic measurements of tissue birefringence: theory and experiments,” Ph.D. dissertation (Northwestern University, Evanston, Ill., 1995).
  21. W. R. Zipfel, R. M. Williams, R. Chritie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, “Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation,” Proc. Nat. Acad. Sci. USA 100, 7075–7080 (2003).
  22. P. J. Campagnola, H. A. Clark, W. A. Mohler, A. Lewis, and L. M. Loew, “Second-harmonic imaging microscopy of living cells,” J. Biomed. Opt. 6, 277–286 (2001).
  23. H. Matsumoto, S. Kitamura, and T. Araki, “Applications of fluorescence microscopy to studies of dental hard tissue,” Front. Med. Biol. Eng. 10, 269–284 (2001).

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