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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 15 — May. 20, 2004
  • pp: 3066–3072

Excitation-emission fluorimeter based on linear interference filters

Michael Gouzman, Nadia Lifshitz, Serge Luryi, Oleg Semyonov, Dmitry Gavrilov, and Vyacheslav Kuzminskiy  »View Author Affiliations


Applied Optics, Vol. 43, Issue 15, pp. 3066-3072 (2004)
http://dx.doi.org/10.1364/AO.43.003066


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Abstract

We describe the design, properties, and performance of an excitation-emission (EE) fluorimeter that enables spectral characterization of an object simultaneously with respect to both its excitation and its emission properties. Such devices require two wavelength-selecting elements, one in the optical path of the excitation broadband light to obtain tunable excitation and the other to analyze the resulting fluorescence. Existing EE instruments are usually implemented with two monochromators. The key feature of our EE fluorimeter is that it employs lightweight and compact linear interference filters (LIFs) as the wavelength-selection elements. The spectral tuning of both the excitation and the detection LIFs is achieved by their mechanical shift relative to each other by use of two computer-controlled linear step motors. The performance of the LIF-based EE fluorimeter is demonstrated with the fluorescent spectra of various dyes and their mixtures.

© 2004 Optical Society of America

OCIS Codes
(300.0300) Spectroscopy : Spectroscopy
(300.6190) Spectroscopy : Spectrometers
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

History
Original Manuscript: October 22, 2003
Revised Manuscript: February 26, 2004
Published: May 20, 2004

Citation
Michael Gouzman, Nadia Lifshitz, Serge Luryi, Oleg Semyonov, Dmitry Gavrilov, and Vyacheslav Kuzminskiy, "Excitation-emission fluorimeter based on linear interference filters," Appl. Opt. 43, 3066-3072 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-15-3066

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