Spectroscopic Studies of Autofluorescence Substances Existing in Human Tissue: Influences of Lactic Acid and Porphyrins
Applied Optics, Vol. 43, Issue 20, pp. 3993-3998 (2004)
http://dx.doi.org/10.1364/AO.43.003993
Acrobat PDF (191 KB)
Abstract
The influence of lactic acid or porphyrins on the optical properties of tissue fluorophores is investigated by autofluorescence (AF) spectroscopy measurement with a GaN-based ultraviolet laser diode along with Fourier-transform IR (FTIR) spectroscopy measurement. As the lactic-acid concentration becomes dense, the AF peak intensity from elastin and desmosine solutions become wholly weak. A similar reduction in the AF intensity is observed for nicotinamide adenine dinucleotide (NADH) solutions. FTIR analysis indicates that the lactic acid causes the conformational change in elastin and the oxidation of NADH, which can be related to changes in the AF properties. The peak intensity of the tissue fluorophores also becomes weak when porphyrins are added, although the conformational change in each tissue fluorophore is not confirmed from FTIR analysis. Judging from the change in the scattering-light intensity of the excitation source, the observed change mainly originates from the absorption of the excitation source by porphyrins.
© 2004 Optical Society of America
OCIS Codes
(160.2540) Materials : Fluorescent and luminescent materials
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.6340) Spectroscopy : Spectroscopy, infrared
Citation
Yasuhiro Ueda and Masakazu Kobayashi, "Spectroscopic Studies of Autofluorescence Substances Existing in Human Tissue: Influences of Lactic Acid and Porphyrins," Appl. Opt. 43, 3993-3998 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-20-3993
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Highly efficient infrared-to-visible energy upconversion in Er3+:Y2O3 (OL)
- Development of high-resolution real-time sub-ppb ethane spectroscopy and some pilot studies in life science (AO)
- Upconversion fluorescence spectroscopy of Er^(3+)-doped lead oxyfluorosilicate glass (COL)
- High speed engine gas thermometry by Fourier-domain mode-locked laser absorption spectroscopy (OE)
- In vivo measurement of absorption coefficient in rat brain and statistic analysis (COL)
Related Conference Papers
- Conformational changes in peptides revealed by time-resolved anisotropy and FRET measurements
- Mid-infrared sensing of carbon monoxide in industrial glass furnaces
- Optical investigations of solid oxide fuel cell modules
- Angleed probe design for scattering measurements from a small tissue volume
- (Yb3+, Er3+) and (Yb3+, Tm3+)-codoped Lu2O3 nanorods: Hydrothermal synthesis and visible emissions
« Previous Article | Next Article »
OSA is a member of 