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Applied Optics

Applied Optics


  • Vol. 43, Iss. 25 — Sep. 1, 2004
  • pp: 4796–4801

Refractometry of microscopic objects with digital holography

Mats Gustafsson and Mikael Sebesta  »View Author Affiliations

Applied Optics, Vol. 43, Issue 25, pp. 4796-4801 (2004)

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Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.

© 2004 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(100.5070) Image processing : Phase retrieval
(110.0180) Imaging systems : Microscopy
(160.4670) Materials : Optical materials
(290.3200) Scattering : Inverse scattering

Original Manuscript: October 24, 2003
Revised Manuscript: March 29, 2004
Manuscript Accepted: May 26, 2004
Published: September 1, 2004

Mats Gustafsson and Mikael Sebesta, "Refractometry of microscopic objects with digital holography," Appl. Opt. 43, 4796-4801 (2004)

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  1. M. Jacquot, P. Sandoz, G. Tribillon, “High resolution digital holography,” Opt. Commun. 190, 87–94 (2001). [CrossRef]
  2. F. Dubois, C. Minetti, O. Monnom, C. Yourassowsky, J. C. Legros, P. Kischel, “Pattern recognition with a digital holographic microscope working in partially coherent illumination,” Appl. Opt. 41, 4108–4119 (2002). [CrossRef] [PubMed]
  3. U. Schnars, W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, R85–R101 (2002). [CrossRef]
  4. S. Seebacher, W. Osten, T. Baumbach, W. Jüptner, “The determination of material parameters of microcomponents using digital holography,” Opt. Lasers Eng. 36, 103–126 (2001). [CrossRef]
  5. W. Xu, M. H. Jericho, I. A. Meinertzhagen, H. J. Kreuzer, “Digital in-line holography for biological applications,” Cell Biol. 98, 11301–11305 (2001).
  6. M. Gustafsson, M. Sebesta, B. Bengtsson, S.-G. Pettersson, P. Egelberg, T. Lenart, “High resolution digital transmission microscopy—a Fourier holography approach,” Opt. Lasers Eng. 41, 553–563 (2004). [CrossRef]
  7. W. D. Nesse, Introduction to Optical Mineralogy, 2nd ed. (Oxford University Press, New York, 1991).
  8. M. Born, E. Wolf, Principles of Optics, 7th ed. (Cambridge University Press, Cambridge, UK, 1999).
  9. D. C. Ghiglia, M. D. Pritt, Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software (Wiley, New York, 1998).

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