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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 25 — Sep. 1, 2004
  • pp: 4831–4837

Optical manipulation in combination with multiphoton microscopy for single-cell studies

Mattias Goksör, Jonas Enger, and Dag Hanstorp  »View Author Affiliations


Applied Optics, Vol. 43, Issue 25, pp. 4831-4837 (2004)
http://dx.doi.org/10.1364/AO.43.004831


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Abstract

We demonstrate how optical tweezers can be incorporated into a multiphoton microscope to achieve three-dimensional imaging of trapped cells. The optical tweezers, formed by a cw 1064 nm Nd:YVO4 laser, were used to trap live yeast cells in suspension while the 4′,6-diamidino-2-phenylindole-stained nucleus was imaged in three dimensions by use of a pulsed femtosecond laser. The trapped cell was moved in the axial direction by changing the position of an external lens, which was used to control the divergence of the trapping laser beam. This gives us a simple method to use optical tweezers in the laser scanning of confocal and multiphoton microscopes. It is further shown that the same femtosecond laser as used for the multiphoton imaging could also be used as laser scissors, allowing us to drill holes in the membrane of trapped spermatozoa.

© 2004 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

History
Original Manuscript: December 16, 2003
Revised Manuscript: May 17, 2004
Manuscript Accepted: May 26, 2004
Published: September 1, 2004

Citation
Mattias Goksör, Jonas Enger, and Dag Hanstorp, "Optical manipulation in combination with multiphoton microscopy for single-cell studies," Appl. Opt. 43, 4831-4837 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-25-4831

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