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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 25 — Sep. 1, 2004
  • pp: 4862–4873

Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids

Ping Yu, Mirela Mustata, Leilei Peng, John J. Turek, Michael R. Melloch, Paul M. W. French, and David D. Nolte  »View Author Affiliations


Applied Optics, Vol. 43, Issue 25, pp. 4862-4873 (2004)
http://dx.doi.org/10.1364/AO.43.004862


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Abstract

Holographic optical coherence imaging is a full-frame variant of coherence-domain imaging. An optoelectronic semiconductor holographic film functions as a coherence filter placed before a conventional digital video camera that passes coherent (structure-bearing) light to the camera during holographic readout while preferentially rejecting scattered light. The data are acquired as a succession of en face images at increasing depth inside the sample in a fly-through acquisition. The samples of living tissue were rat osteogenic sarcoma multicellular tumor spheroids that were grown from a single osteoblast cell line in a bioreactor. Tumor spheroids are nearly spherical and have radial symmetry, presenting a simple geometry for analysis. The tumors investigated ranged in diameter from several hundred micrometers to over 1 mm. Holographic features from the tumors were observed in reflection to depths of 500–600 μm with a total tissue path length of approximately 14 mean free paths. The volumetric data from the tumor spheroids reveal heterogeneous structure, presumably caused by necrosis and microcalcifications characteristic of some human avascular tumors.

© 2004 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.4500) Imaging systems : Optical coherence tomography
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(190.5330) Nonlinear optics : Photorefractive optics
(190.5970) Nonlinear optics : Semiconductor nonlinear optics including MQW

History
Original Manuscript: December 15, 2003
Manuscript Accepted: March 12, 2004
Published: September 1, 2004

Citation
Ping Yu, Mirela Mustata, Leilei Peng, John J. Turek, Michael R. Melloch, Paul M. W. French, and David D. Nolte, "Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids," Appl. Opt. 43, 4862-4873 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-25-4862

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