A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum’s peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 μm at a wavelength of 0.633 μm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 μm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others.
© 2004 Optical Society of America
(100.3190) Image processing : Inverse problems
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.0290) Scattering : Scattering
(290.5850) Scattering : Scattering, particles
Konstantin A. Semyanov, Peter A. Tarasov, Alexey E. Zharinov, Andrei V. Chernyshev, Alfons G. Hoekstra, and Valeri P. Maltsev, "Single-Particle Sizing from Light Scattering by Spectral Decomposition," Appl. Opt. 43, 5110-5115 (2004)