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Applied Optics

Applied Optics


  • Vol. 43, Iss. 26 — Sep. 10, 2004
  • pp: 5110–5115

Single-particle sizing from light scattering by spectral decomposition

Konstantin A. Semyanov, Peter A. Tarasov, Alexey E. Zharinov, Andrei V. Chernyshev, Alfons G. Hoekstra, and Valeri P. Maltsev  »View Author Affiliations

Applied Optics, Vol. 43, Issue 26, pp. 5110-5115 (2004)

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A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum’s peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 μm at a wavelength of 0.633 μm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 μm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others.

© 2004 Optical Society of America

OCIS Codes
(100.3190) Image processing : Inverse problems
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.0290) Scattering : Scattering
(290.5850) Scattering : Scattering, particles

Original Manuscript: October 23, 2003
Revised Manuscript: May 7, 2004
Published: September 10, 2004

Konstantin A. Semyanov, Peter A. Tarasov, Alexey E. Zharinov, Andrei V. Chernyshev, Alfons G. Hoekstra, and Valeri P. Maltsev, "Single-particle sizing from light scattering by spectral decomposition," Appl. Opt. 43, 5110-5115 (2004)

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