<i>In vitro</i> experiments have demonstrated the ability of Raman spectroscopy to diagnose a wide variety of diseases. Recent <i>in vivo</i> investigations performed with optical fiber probes were promising but generally limited to easily accessible organs, often requiring relatively long collection times. We have implemented an optical design strategy to utilize system throughput fully by characterizing the Raman distribution from tissue. This scheme optimizes collection efficiency, minimizes noise, and has resulted in small-diameter, highly efficient Raman probes that are capable of collecting high-quality data in 1 s. Performance has been tested through simulations and experiments with tissue models and several <i>in vitro</i> tissue types, demonstrating that this new design can advance Raman spectroscopy as a clinically practical technique.
© 2004 Optical Society of America
(060.2310) Fiber optics and optical communications : Fiber optics
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.4570) Instrumentation, measurement, and metrology : Optical design of instruments
(170.5660) Medical optics and biotechnology : Raman spectroscopy
Jason T. Motz, Martin Hunter, Luis H. Galindo, Joseph A. Gardecki, John R. Kramer, Ramachandra R. Dasari, and Michael S. Feld, "Optical Fiber Probe for Biomedical Raman Spectroscopy," Appl. Opt. 43, 542-554 (2004)