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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 3 — Jan. 20, 2004
  • pp: 564–574

Phosphorescence lifetime imaging in turbid media: the inverse problem and experimental image reconstruction

Vadim Y. Soloviev, David F. Wilson, and Sergei A. Vinogradov  »View Author Affiliations


Applied Optics, Vol. 43, Issue 3, pp. 564-574 (2004)
http://dx.doi.org/10.1364/AO.43.000564


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Abstract

Three-dimensional phosphorescence lifetime imaging is a novel method for the mapping of oxygen concentration in biological tissues. We present reconstruction techniques for recovering phosphorescent objects in highly scattering media based on the telegraph equation and two regularization methods, i.e., the Tikhonov—Phillips regularization and the maximum entropy method. Theoretical results are experimentally validated, and the reconstructed images of phosphorescent objects rendering oxygen maps in a layer are presented.

© 2004 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(290.0290) Scattering : Scattering
(290.7050) Scattering : Turbid media

History
Original Manuscript: June 30, 2003
Revised Manuscript: October 10, 2003
Published: January 20, 2004

Citation
Vadim Y. Soloviev, David F. Wilson, and Sergei A. Vinogradov, "Phosphorescence lifetime imaging in turbid media: the inverse problem and experimental image reconstruction," Appl. Opt. 43, 564-574 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-3-564

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