OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 43, Iss. 36 — Dec. 20, 2004
  • pp: 6536–6544

Parameter-optimized digital holographic microscope for high-resolution living-cell analysis

Daniel Carl, Björn Kemper, Günther Wernicke, and Gert von Bally  »View Author Affiliations

Applied Optics, Vol. 43, Issue 36, pp. 6536-6544 (2004)

View Full Text Article

Enhanced HTML    Acrobat PDF (910 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A parameter-optimized off-axis setup for digital holographic microscopy is presented for simultaneous, high-resolution, full-field quantitative amplitude and quantitative phase-contrast microscopy and the detection of changes in optical path length in transparent objects, such as undyed living cells. Numerical reconstruction with the described nondiffractive reconstruction method, which suppresses the zero order and the twin image, requires a mathematical model of the phase-difference distribution between the object wave and the reference wave in the hologram plane. Therefore an automated algorithm is explained that determines the parameters of the mathematical model by carrying out the discrete Fresnel transform. Furthermore the relationship between the axial position of the object and the reconstruction distance, which is required for optimization of the lateral resolution of the holographic images, is derived. The lateral and the axial resolutions of the system are discussed and quantified by application to technical objects and to living cells.

© 2004 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy

Original Manuscript: March 5, 2004
Revised Manuscript: July 3, 2004
Manuscript Accepted: September 13, 2004
Published: December 20, 2004

Daniel Carl, Björn Kemper, Günther Wernicke, and Gert von Bally, "Parameter-optimized digital holographic microscope for high-resolution living-cell analysis," Appl. Opt. 43, 6536-6544 (2004)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. Kreis, Holographic Interferometry, Principles and Methods: Diffraction Theory (Akademie Verlag GmbH, Berlin, 1996), pp. 27–31.
  2. U. Schnars, “Direct phase determination in hologram interferometry with use of digitally recorded holograms,” J. Opt. Soc. Am. A 11, 2011–2015 (1994). [CrossRef]
  3. G. Pedrini, P. Fröning, H. J. Tiziani, F. M. Santoyo, “Shape measurement of microscopic structures using digital holograms,” Opt. Commun. 164, 257–268 (1999). [CrossRef]
  4. E. Cuche, P. Marquet, P. Magistretti, C. Depeursinge, “Quantitative phase contrast microscopy of living cells by numerical reconstruction of digital holograms,” in Optical Diagnostics of Living Cells II, D. L. Farkas, R. C. Leif, B. J. Tromberg, eds., Proc. SPIE3604, 84–89 (1999). [CrossRef]
  5. E. Cuche, P. Marquet, C. Depeursinge, “Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms,” Appl. Opt. 38, 6994–7001 (1999). [CrossRef]
  6. U. Schnars, W. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13, 85–101 (2002). [CrossRef]
  7. S. Lai, B. Kemper, G. v. Bally, “Off-axis reconstruction of in-line holograms for twin-image elimination,” Opt. Commun. 169, 37–43 (1999). [CrossRef]
  8. L. Xu, J. Miao, A. Asundi, “Properties of digital holography based on in-line configuration,” Opt. Eng. 39, 3214–3219 (2000). [CrossRef]
  9. W. Xu, M. H. Jerico, I. A. Meinertzhagen, H. J. Kreuzner, “Digital in-line holography of microspheres,” Appl. Opt. 41, 5367–5375 (2002). [CrossRef] [PubMed]
  10. M. Liebling, T. Blu, E. Cuche, P. Marquet, C. Depeursinge, M. Unser, “A novel nondiffractive reconstruction method for digital holographic microscopy,” in Proceedings of IEEE International Symposium on Biomedical Imaging 2002 (Institute of Electrical and Electronics Engineers, New York, 2002), Vol. 2, pp. 625–628. [CrossRef]
  11. H. A. Aebischer, S. Waldner, “A simple and effective method for filtering speckle-interferometric phase fringe patterns,” Opt. Commun. 162, 205–210 (1999). [CrossRef]
  12. T. Kreis, Holographic Interferometry, Principles and Methods: Interference Phase Demodulation (Akademie Verlag GmbH, Berlin, 1996, pp. 161–170.
  13. S. Inoué, K. Spring, Video Microscopy: The Fundamentals (Plenum, New York, 1997). [CrossRef]
  14. T. Kreis, Holographic Interferometry, Principles and Methods: Principle of Digital Holography (Akademie Verlag GmbH, Berlin, 1996), pp. 150–153.
  15. B. Kemper, J. Kandulla, D. Dirksen, G. v. Bally, “Optimization of spatial phase shifting in endoscopic electronic speckle pattern interferometry,” Opt. Commun. 217, 151–160 (2003). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited