Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.
© 2004 Optical Society of America
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
(220.0220) Optical design and fabrication : Optical design and fabrication
(220.4830) Optical design and fabrication : Systems design
Andrew Ridsdale, Ileana Micu, and Peter K. Stys, "Conversion of the Nikon C1 Confocal Laser-Scanning Head for Multiphoton Excitation on an Upright Microscope," Appl. Opt. 43, 1669-1675 (2004)