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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 43, Iss. 8 — Mar. 10, 2004
  • pp: 1669–1675

Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope

Andrew Ridsdale, Ileana Micu, and Peter K. Stys  »View Author Affiliations


Applied Optics, Vol. 43, Issue 8, pp. 1669-1675 (2004)
http://dx.doi.org/10.1364/AO.43.001669


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Abstract

Most commercial laser-scanning imaging systems used for confocal fluorescence microscopy can be readily adapted for use with two-photon fluorescence excitation. We report here on the details of the conversion of the Nikon C1 (product released November 2001) with two channels of nondescanned detection of two-photon-excited fluorescence. One of the goals of the design was to utilize off-the-shelf components as much as possible to minimize the use of custom machining and electronics assembly. We also give some initial characterization of the imaging properties of the system.

© 2004 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
(220.0220) Optical design and fabrication : Optical design and fabrication
(220.4830) Optical design and fabrication : Systems design

History
Original Manuscript: May 6, 2003
Revised Manuscript: December 15, 2003
Published: March 10, 2004

Citation
Andrew Ridsdale, Ileana Micu, and Peter K. Stys, "Conversion of the Nikon C1 confocal laser-scanning head for multiphoton excitation on an upright microscope," Appl. Opt. 43, 1669-1675 (2004)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-43-8-1669


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