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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 10 — Apr. 1, 2005
  • pp: 1823–1830

Determination of optimal exposure time for imaging of blood flow changes with laser speckle contrast imaging

Shuai Yuan, Anna Devor, David A. Boas, and Andrew K. Dunn  »View Author Affiliations


Applied Optics, Vol. 44, Issue 10, pp. 1823-1830 (2005)
http://dx.doi.org/10.1364/AO.44.001823


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Abstract

Laser speckle contrast imaging is becoming an established method for full-field imaging of cerebral blood flow dynamics in animal models. The sensitivity and noise in the measurement of blood flow changes depend on the camera exposure time. The relation among sensitivity, noise, and camera exposure time was investigated experimentally by imaging the speckle contrast changes in the brain after electrical forepaw stimulation in rats. The sensitivity to relative changes in speckle contrast was found to increase at longer exposure times and to reach a plateau for exposure times greater than approximately 2 ms. However, the speckle contrast noise also increases with exposure time and thus the contrast-to-noise ratio was found to peak at an exposure time of approximately 5 ms. Our results suggests that ~5 ms is an optimal exposure time for imaging of stimulus-induced changes in cerebral blood flow in rodents.

© 2005 Optical Society of America

OCIS Codes
(120.6150) Instrumentation, measurement, and metrology : Speckle imaging
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

Citation
Shuai Yuan, Anna Devor, David A. Boas, and Andrew K. Dunn, "Determination of optimal exposure time for imaging of blood flow changes with laser speckle contrast imaging," Appl. Opt. 44, 1823-1830 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-10-1823


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References

  1. M. Englehart and J. K. Kristensen, "Evaluation of cutaneous blood flow response by Xenon washout and a laser Doppler flowmeter," J. Invest. Dermatol.  80, 12-15 (1983).
  2. A. V. J. Challoner, "Photoelectric plethysmography for estimating cutaneous blood flow," in Non-invasive physiological measurements, P. Rolfe, ed. (Academic, London, 1979), Vol. 1, pp. 125-151.
  3. K. R. Forrester, C. Stewart, J. Tulip, C. Leonard, and R. C. Bray, "Comparison of laser speckle and laser Doppler perfusion imaging: measurement in human skin and rabbit articular tissue," Med. Biol. Eng. Comput.  40, 687-697 (2002).
  4. A. F. Fercher and J. D. Briers, "Flow visualization by means of single-exposure speckle photography," Opt. Commun.  37, 326-330 (1981).
  5. J. D. Briers, "Laser Doppler, speckle and related techniques for blood perfusion mapping and imaging," Physiol. Meas.  22, R35-R66 (2001).
  6. J. D. Briers and A. F. Fercher, "Retinal blood-flow visualization by means of single-exposure speckle photography," Invest. Ophthalmol. Vis. Sci.  22, 255-259 (1982).
  7. J. D. Briers and S. Webster, "Quasi-real time digital version of single-exposure speckle photography for full-field monitoring of velocity or flow fields," Opt. Commun.  116, 36-42 (1995).
  8. J. D. Briers and S. Webster, "Laser speckle contrast analysis (LASCA): a non-scanning, full-field technique for monitoring capillary blood flow," J. Biomed. Opt.  1, 174-179 (1996).
  9. J. D. Briers, G. Richards, and X. W. He, "Capillary blood flow monitoring using laser speckle contrast analysis (LASCA)," J. Biomed. Opt.  4, 164-175 (1999).
  10. A. K. Dunn, H. Bolay, M. A. Moskowitz, and D. A. Boas, "Dynamic imaging of cerebral blood flow using laser speckle," J. Cereb. Blood Flow Metab.  21, 195-201 (2001).
  11. H. Fujii, "Visualization of retinal blood flow by laser speckle flowgraphy," Med. Biol. Eng. Comput.  32, 302-304 (1994).
  12. N. Konishi and H. Fujii, "Real-time visualization of retinal microcirculation by laser flowgraphy," Opt. Eng.  34, 753-757 (1995).
  13. Y. Aizu, T. Asakura, and A. Kojima, "Compensation of eye movements in retinal speckle flowmetry using flexible correlation analysis based on the specific variance," J. Biomed. Opt.  3, 227-236 (1998).
  14. B. Choi, N. M. Kang, and J. S. Nelson, "Laser speckle imaging for monitoring blood flow dynamics in the in vivo rodent dorsal skin fold model," Microvasc. Res.  68, 143-146 (2004).
  15. G. J. Tearney and B. E. Bouma, "Atherosclerotic plaque characterization by spatial and temporal speckle pattern analysis," Opt. Lett.  27, 533-535 (2002).
  16. A. K. Dunn, A. Devor, H. Bolay, M. L. Andermann, M. A. Moskowitz, A. M. Dale, and D. A. Boas, "Simultaneous imaging of total cerebral hemoglobin concentration, oxygenation, and blood flow during functional activation," Opt. Lett.  28, 28-30 (2003).
  17. H. Bolay, U. Reuter, A. K. Dunn, Z. Huang, D. A. Boas, and M. A. Moskowitz, "Intrinsic brain activity triggers trigeminal meningeal afferents in a migraine model," Nat. Med.  8, 36-42 (2002).
  18. A. K. Dunn, A. Devor, H. Bolay, M. L. Andermann, M. A. Moskowitz, A. M. Dale, and D. A. Boas, "Simultaneous imaging of total cerebral hemoglobin concentration, oxygenation, and blood flow during functional activation," Opt. Lett.  28, 28-30 (2003).
  19. T. Durduran, M. G. Burnett, G. Yu, C. Zhou, D. Furuya, A. G. Yodh, J. A. Detre, and J. H. Greenberg, "Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry," J. Cereb. Blood Flow Metab.  24, 518-525 (2004).
  20. C. Ayata, A. K. Dunn, Y. Gursoy-Ozdemir, Z. Huang, D. A. Boas, and M. A. Moskowitz, "Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex," J. Cereb. Blood Flow Metab.  24, 744-755 (2004).
  21. D. N. Atochin, J. C. Murciano, Y. Gursoy-Ozdemir, T. Krasik, F. Noda, C. Ayata, A. K. Dunn, M. A. Moskowitz, P. L. Huang, and V. R. Muzykantov, "Mouse model of microembolic stroke and reperfusion," Stroke  35, 2177-2182 (2004).
  22. S. C. Jones, K. A. Easley, C. R. Radinsky, D. Chyatte, A. J. Furlan, and A. D. Perez-Trepichio, "Nitric oxide synthase inhibition depresses the height of the cerebral blood flow-pressure autoregulation curve during moderate hypotension," J. Cereb. Blood Flow Metab.  23, 1085-1095 (2003).
  23. J. W. Goodman, "Statistical properties of laser speckle patterns," in Laser Speckle and Related Topics, 2nd ed.J.C.Dainty, ed. (Springer, Berlin, 1975), pp. 9-75.
  24. R. Bonner and R. Nossal, "Model for laser Doppler measurements of blood flow in tissue," Appl. Opt.  20, 2097-2107 (1981).
  25. J. F. Kenney and E. S. Keeping, "The distribution of the standard deviation," in Mathematics of Statistics, Pt. 2, 2nd ed. (Van Nostrand, Princeton, N.J., 1951), pp. 170-173.

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