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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 10 — Apr. 1, 2005
  • pp: 1845–1849

Experimental measurement of the refractive index of biological tissues by total internal reflection

Jiancheng Lai, Zhenhua Li, Chunyong Wang, and Anzhi He  »View Author Affiliations


Applied Optics, Vol. 44, Issue 10, pp. 1845-1849 (2005)
http://dx.doi.org/10.1364/AO.44.001845


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Abstract

We discuss the refractive-index measurement of biological tissues by total internal reflection. The methodology of the measurement is illuminated comprehensively, and an experimental setup, combined with a data processing program, is developed correspondingly. Refractive indices of typical tissue samples are measured by use of the developed methodology. The agreement of our measurements with the reported results shows the validity of our scheme, which has the potential for being a simple, quick, and low-cost practical means for determining the refractive index of a turbid medium. Moreover, an empirical formula for evaluating the refractive index of Intralipid suspensions with different concentrations is also presented according to experimental measurements.

© 2005 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

History
Original Manuscript: August 18, 2004
Revised Manuscript: November 6, 2004
Manuscript Accepted: November 11, 2004
Published: April 1, 2005

Citation
Jiancheng Lai, Zhenhua Li, Chunyong Wang, and Anzhi He, "Experimental measurement of the refractive index of biological tissues by total internal reflection," Appl. Opt. 44, 1845-1849 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-10-1845

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