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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 10 — Apr. 1, 2005
  • pp: 1845–1849

Experimental measurement of the refractive index of biological tissues by total internal reflection

Jiancheng Lai, Zhenhua Li, Chunyong Wang, and Anzhi He  »View Author Affiliations


Applied Optics, Vol. 44, Issue 10, pp. 1845-1849 (2005)
http://dx.doi.org/10.1364/AO.44.001845


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Abstract

We discuss the refractive-index measurement of biological tissues by total internal reflection. The methodology of the measurement is illuminated comprehensively, and an experimental setup, combined with a data processing program, is developed correspondingly. Refractive indices of typical tissue samples are measured by use of the developed methodology. The agreement of our measurements with the reported results shows the validity of our scheme, which has the potential for being a simple, quick, and low-cost practical means for determining the refractive index of a turbid medium. Moreover, an empirical formula for evaluating the refractive index of Intralipid suspensions with different concentrations is also presented according to experimental measurements.

© 2005 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

History
Original Manuscript: August 18, 2004
Revised Manuscript: November 6, 2004
Manuscript Accepted: November 11, 2004
Published: April 1, 2005

Citation
Jiancheng Lai, Zhenhua Li, Chunyong Wang, and Anzhi He, "Experimental measurement of the refractive index of biological tissues by total internal reflection," Appl. Opt. 44, 1845-1849 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-10-1845


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References

  1. M. Motamedi, S. Rastegar, “Light and temperature distribution in laser irradiated tissue: the influence of anisotropic scattering and refractive index,” Appl. Opt. 28, 2230–2237 (1989). [CrossRef] [PubMed]
  2. S. R. Arridge, J. C. Hebden, “Optical imaging in medicine: II. Modelling and reconstruction,” Phys. Med. Biol. 42, 841–853 (1997). [CrossRef] [PubMed]
  3. A. Knuttel, M. Boehlau-Godau, “Spatially confined and temporally resolved refractive index and scattering evaluation in human skin performed with optical coherence tomography,” J. Biomed. Opt. 5, 83–92 (2000). [CrossRef] [PubMed]
  4. F. Bolin, L. Preuss, R. Taylor, R. Ference, “Refractive index of some mammalian tissues using a fiber optics cladding method,” Appl. Opt. 28, 2297–2303 (1989). [CrossRef] [PubMed]
  5. H. Li, S. Xie, “Measurement method of the refractive index of biotissue by total internal reflection,” Appl. Opt. 35, 1793–1795 (1996). [CrossRef] [PubMed]
  6. G. J. Tearney, M. E. Brezinski, J. F. Southern, B. E. Bouma, M. R. Hee, J. G. Fujimoto, “Determination of the refractive index of highly scattering human tissue by optical coherence tomography,” Opt. Lett. 20, 2258–2260 (1995). [CrossRef] [PubMed]
  7. S. A. Alexandrov, A. V. Zvyagin, K. K. M. B. D. Silva, D. D. Sampson, “Bifocal optical coherence refractometry of turbid media,” Opt. Lett. 28, 117–119 (2003). [CrossRef] [PubMed]
  8. J. M. Schmitt, G. Kumar, “Turbulent nature of refractive-index variations in biological tissue,” Opt. Lett. 21, 1310–1312 (1996). [CrossRef] [PubMed]
  9. J. Rheims, J. Kosery, T. Wriedt, “Refractive-index measurements in the near-IR using an Abbe refractometer,” Meas. Sci. Technol. 8, 601–605 (1997). [CrossRef]
  10. S. T. Flock, S. L. Jacques, B. C. Wilson, W. M. Star, “Optical properties of Intralipid: a phantom medium for light propagation studies,” Lasers Surg. Med. 12, 510–519 (1992). [CrossRef] [PubMed]
  11. V. V. Tuchin, “Tissue image contrasting using optical immersion technique,” in Biomedical Photonics and Optoelectronic Imaging, H. Liu, Q. Luo, eds., Proc. SPIE4224, 351–365 (2000). [CrossRef]

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