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Applied Optics

Applied Optics


  • Vol. 44, Iss. 10 — Apr. 1, 2005
  • pp: 1905–1916

Time-resolved diffuse optical tomographic imaging for the provision of both anatomical and functional information about biological tissue

Huijuan Zhao, Feng Gao, Yukari Tanikawa, Kazuhiro Homma, and Yukio Yamada  »View Author Affiliations

Applied Optics, Vol. 44, Issue 10, pp. 1905-1916 (2005)

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We present in vivo images of near-infrared (NIR) diffuse optical tomography (DOT) of human lower legs and forearm to validate the dual functions of a time-resolved (TR) NIR DOT in clinical diagnosis, i.e., to provide anatomical and functional information simultaneously. The NIR DOT system is composed of time-correlated single-photon-counting channels, and the image reconstruction algorithm is based on the modified generalized pulsed spectral technique, which effectively incorporates the TR data with reasonable computation time. The reconstructed scattering images of both the lower legs and the forearm revealed their anatomies, in which the bones were clearly distinguished from the muscles. In the absorption images, some of the blood vessels were observable. In the functional imaging, a subject was requested to do handgripping exercise to stimulate physiological changes in the forearm tissue. The images of oxyhemoglobin, deoxyhemoglobin, and total hemoglobin concentration changes in the forearm were obtained from the differential images of the absorption at three wavelengths between the exercise and the rest states, which were reconstructed with a differential imaging scheme. These images showed increases in both blood volume and oxyhemoglobin concentration in the arteries and simultaneously showed hypoxia in the corresponding muscles. All the results have demonstrated the capability of TR NIR DOT by reconstruction of the absolute images of the scattering and the absorption with a high spatial resolution that finally provided both the anatomical and functional information inside bulky biological tissues.

© 2005 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.6960) Medical optics and biotechnology : Tomography

Original Manuscript: July 20, 2004
Revised Manuscript: October 28, 2004
Manuscript Accepted: November 1, 2004
Published: April 1, 2005

Huijuan Zhao, Feng Gao, Yukari Tanikawa, Kazuhiro Homma, and Yukio Yamada, "Time-resolved diffuse optical tomographic imaging for the provision of both anatomical and functional information about biological tissue," Appl. Opt. 44, 1905-1916 (2005)

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  1. C. H. Schmitz, H. L. Graber, H. Luo, R. L. Barbour, Y. Pei, S. Zhong, “Instrumentation and calibration protocol for imaging dynamic features in dense-scattering media by optical tomography,” Appl. Opt. 39, 6466–6486 (2000). [CrossRef]
  2. G. Strangman, D. A. Boas, J. Sutton, “Non-invasive neuro-imaging using near infrared light,” Biol. Psychiatry 52, 679–693 (2002). [CrossRef] [PubMed]
  3. H. Eda, I. Oda, Y. Ito, Y. Wada, Y. Oikawa, Y. Tsunazawa, M. Takada, Y. Tsuchiya, Y. Yamashita, M. Oda, A. Sassaroli, Y. Yamada, M. Tamura, “Multichannel time-resolved optical tomographic imaging system,” Rev. Sci. Instrum. 70, 3595–3601 (1999). [CrossRef]
  4. E. M. C. Hillman, J. C. Hebden, M. Schweiger, H. Dehghani, F. E. W. Schmidt, D. T. Delpy, S. R. Arridge, “Time resolved optical tomography of the human forearm,” Phys. Med. Biol. 46, 1117–1130 (2001). [CrossRef] [PubMed]
  5. R. Cubeddu, A. Pifferi, L. Spinelli, P. Taroni, A. Torricelli, “Breast lesion characterization by a novel nonlinear perturbation approach,” in Photon Migration and Diffuse-Light Imaging,D. A. Boas ed., Proc. SPIE5138, 23–29 (2003). [CrossRef]
  6. S. Fantini, S. A. Walker, M. A. Franceschini, M. Kaschke, P. M. Schlag, K. T. Moesta, “Assessment of the size, position, and optical properties of breast tumors in vivo by noninvasive optical methods,” Appl. Opt. 37, 1982–1989 (1998). [CrossRef]
  7. B. W. Pogue, M. Testorf, T. McBridge, U. Osterberg, K. Paulsen, “Instrumentation and design of a frequency domain diffuse optical tomographic imager for breast cancer detection,” Opt. Express1, 391–403 (1997), http://www.opticsexpress.org . [CrossRef]
  8. T. O. McBride, B. W. Pogue, E. D. Gerety, S. B. Poplack, U. L. Osterberg, K. D. Paulsen, “Spectroscopic diffuse optical tomography for the quantitative assessment of hemoglobin concentration and oxygen saturation in breast tissue,” Appl. Opt. 38, 5480–5490 (1999). [CrossRef]
  9. D. Grosenick, H. Wabnitz, H. Rinneberg, K. T. Moesta, P. M. Schlag, “Development of a time-resolved optical mammography and first in vivo applications,” Appl. Opt. 38, 2927–2943 (1999). [CrossRef]
  10. J. C. Hebden, A. Gibson, R. M. Yusof, N. Everdell, E. M. C. Hillman, D. T. Delpy, S. R. Arridge, T. Austin, J. H. Meek, J. S. Wyatt, “Three-dimensional optical tomography of the premature infant brain,” Phys. Med. Biol. 47, 4155–4166 (2002). [CrossRef] [PubMed]
  11. J. C. Hebden, A. Gibson, T. Austin, R. M. Yusof, N. Everdell, D. T. Delpy, S. R. Arridge, J. H. Meek, J. S. Wyatt, “Imaging changes in blood volume and oxygenation in the newborn infant brain using three-dimensional optical tomography,” Phys. Med. Biol. 49, 1117–1130 (2004). [CrossRef] [PubMed]
  12. A. Villringer, B. Chance, “Noninvasive optical spectroscopy and imaging of human brain function,” Trends Neurosci. 20, 435–442 (1997). [CrossRef] [PubMed]
  13. D. A. Boas, D. H. Brooks, C. A. Dimarzio, M. Kilmer, R. J. Gaudette, Q. Zhang, “Imaging the body with diffuse optical tomography,” IEEE Signal Processing Mag. 11, 57–75 (2001). [CrossRef]
  14. F. Gao, H. Zhao, Y. Yamada, “Improvement of image quality in diffuse optical tomography by use of full time-resolved data,” Appl. Opt. 41, 778–791 (2002). [CrossRef] [PubMed]
  15. F. Gao, P. Poulet, Y. Yamada, “Simultaneous mapping of absorption and scattering coefficients from a three-dimensional model of time-resolved optical tomography,” App. Opt. 39, 5898–5910 (2001). [CrossRef]
  16. S. R. Arridge, “Optical tomography in medical imaging,” Inverse Probl. 15, R41–93 (1999). [CrossRef]
  17. H. Zhao, F. Gao, Y. Tanikawa, Y. Onodera, M. Ohmi, M. Haruna, Y. Yamada, “Imaging of in vitro chicken leg by use of time-resolved near infrared optical tomography,” Phys. Med. Biol. 47, 1979–1993 (2002). [CrossRef] [PubMed]
  18. R. Cubeddu, G. Biscoti, A. Pifferi, P. Taroni, A. Torricelli, M. Ferrari, V. Quaresima, “Functional muscle studies by dual-wavelength, 8 channel time-resolved oximetry,” in Photon Migration and Diffuse-Light Imaging, D. Boas ed., Proc. SPIE5138, 29–34 (2003). [CrossRef]
  19. M. Klasing, J. Zange, “In vivo quantitative near-infrared spectroscopy in skeletal muscle and bone during rest and isometric exercise,” in Photon Migration and Diffuse-Light Imaging, D. Boas ed., Proc. SPIE5138, 318–322 (2003). [CrossRef]
  20. M. C. P. van Beekvelt, K. Orbon, B. G. M. van Engelen, R. A. Wevers, W. N. J. M. Colier, “NIR spectroscopic measurement of local muscle metabolism during rhythmic, sustained and intermittent handgrip exercise,” in Photon Migration and Diffuse-Light Imaging, D. Boas ed., Proc. SPIE5138, 35–45 (2003). [CrossRef]
  21. F. Gao, H. Zhao, Y. Tanikawa, Y. Yamada, “Time-resolved diffuse optical tomography using a modified generalized pulse spectrum technique,” IEICE Trans. Inf. Syst. E85-D, 133–142 (2002).
  22. K. Furutsu, Y. Yamada, “Diffusion approximation for a dissipative random medium and the application,” Phys. Rev. E 50, 3634–3640 (1994). [CrossRef]
  23. W. G. Egan, T. W. Hilgeman, eds., Optical Properties of Inhomogeneous Materials (Academic, New York, 1979).
  24. M. Schweiger, S. R. Arridge, M. Hiraoka, D. T. Delpy, “The finite element model for the propagation of light in scattering media: boundary and source conditions,” Med. Phys. 22, 1779–1792 (1995). [CrossRef] [PubMed]
  25. S. R. Arridge, “Photon measurement density functions. Part 1: Analytic forms,” Appl. Opt. 34, 7395–7409 (1995). [CrossRef] [PubMed]
  26. S. R. Arridge, M. Schweiger, “Photon measurement density functions. Part 2: Finite element calculations,” Appl. Opt. 34, 8026–8037 (1995). [CrossRef] [PubMed]
  27. A. Matsukawa, T. Ito, K. Kimura, Transverse Anatomy of Human Body for Diagnosis with Imaging (Igaku Tosho Shuppan Co., LTD., Tokyo, Japan, 1987; in Japanese).
  28. I. Konish, S. Takeuchi, Y. Oikawa, Y. Wada, N. Sakauchi, Y. Ito, I. Oda, Y. Tsunazawa, “Development of OMM-2000 Optical Multi-channel Monitor,” Shimadzu Rev. 57, 141–146 (2000, in Japanese).
  29. F. Gao, H. Zhao, Y. Onodera, A. Sassaroli, Y. Tanikawa, Y. Yamada, “Image reconstruction from experimental measurements of an multichannel time resolved optical tomographic imaging system,” in Optical Tomography and Spectroscopy of Tissue IV,B. Chance, R. R. Alfano, B. J. Tromberg, M. Tamura, E. M. Servick-Muraca eds., Proc. SPIE4250, 351–361 (2001). [CrossRef]
  30. H. Zhao, F. Gao, Y. Tanikawa, K. Homma, Y. Onodera, Y. Yamada, “Anatomical and functional images of in vitro and in vivo tissues by NIR time-domain diffuse optical tomography,” JSME Int. J. Ser. C 45, 1979–1993 (2002). [CrossRef]
  31. G. Zaccanti, A. Taddeucci, M. Barilli, P. Bruscaglioni, F. Martilli, “Optical properties of biological tissues,” in Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation, B. Chance, R. R. Alfano, A. Katzir eds., Proc. SPIE2389, 513–521 (1995). [CrossRef]
  32. C. R. Simpson, M. Kohl, M. Essenpreis, M. Cope, “Near infrared optical properties of ex vivo human skin and subcutaneous tissues measured using the Monte Carlo inversion technique,” Phys. Med. Biol. 43, 2465–2478 (1998). [CrossRef] [PubMed]
  33. S. J. Matcher, M. Cope, D. T. Delpy, “In vivo measurements of the wavelength dependence of tissue-scattering coefficients between 760 and 900 nm measured with time-resolved spectroscopy,” Appl. Opt. 36, 386–396 (1997). [CrossRef] [PubMed]
  34. M. Firbank, M. Hiraoka, M. Essenpreis, D. T. Delpy, “Measurement of the optical properties of the skull in the wavelength range 650–950 nm,” Phys. Med. Biol. 38, 503–510 (1993). [CrossRef] [PubMed]
  35. F. Bevilacqua, D. Piguet, P. Marquet, J. D. Gross, B. J. Thromberg, C. Depeursinge, “In vivo local determination of tissue optical properties: applications to human brain,” Appl. Opt. 38, 4939–4950 (1999). [CrossRef]
  36. H. Zhao, “Theoretical and experimental studies on near infrared time-resolved diffuse optical imaging,” Ph.D. dissertation (The University of Electro-Communications, Japan, 2004).
  37. L. A. Paunescu, “Tissue blood flow and oxygen consumption measured with near-infrared frequency-domain spectroscopy,” Ph.D. thesis (University Illinois at Urbana-Champaign, Ill., 2001).
  38. S. Homma, H. Eda, S. Ogasawara, A. Kagaya, “Near infrared estimation of O2 supply and consumption in forearm muscles working at varying intensity,” J. Appl. Physiol. 80, 1279–1284 (1996).

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