OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 11 — Apr. 10, 2005
  • pp: 2001–2007

Optical micromanipulations inside yeast cells

Leonardo Sacconi, Iva M. Tolić-Nørrelykke, Chiara Stringari, Renzo Antolini, and Francesco S. Pavone  »View Author Affiliations


Applied Optics, Vol. 44, Issue 11, pp. 2001-2007 (2005)
http://dx.doi.org/10.1364/AO.44.002001


View Full Text Article

Enhanced HTML    Acrobat PDF (1885 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a combination of nonlinear microscopy and optical trapping applied to three-dimensional imaging and manipulation of intracellular structures in living cells. We use Titanium-sapphire laser pulses for nonlinear microscopy of the nuclear envelope and the microtubules marked with green fluorescent protein in fission yeast. The same laser source is also used to trap small lipid granules naturally present in the cell. The trapped granule is used as a handle to exert a pushing force on the cell nucleus. The granule is moved in a raster-scanning fashion to cover the area of the nucleus and hence displace the nucleus away from its normal position in the center of the cell. Such indirect manipulations of an organelle (e.g., nucleus) can be useful when direct trapping of the chosen organelle is disadvantageous or inefficient. We show that nonlinear microscopy and optical manipulation can be performed without substantial damage or heating of the cell. We present this method as an important tool in cell biology for manipulation of specific structures, as an alternative to genetic and biochemical methods. This technique can be applied to several fundamental problems in cell biology, including the mechanism of nuclear positioning and the spatial coordination of nuclear and cell division.

© 2005 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(180.5810) Microscopy : Scanning microscopy
(190.0190) Nonlinear optics : Nonlinear optics

History
Original Manuscript: July 19, 2004
Revised Manuscript: November 2, 2004
Manuscript Accepted: November 19, 2004
Published: April 10, 2005

Citation
Leonardo Sacconi, Iva M. Tolić-Nørrelykke, Chiara Stringari, Renzo Antolini, and Francesco S. Pavone, "Optical micromanipulations inside yeast cells," Appl. Opt. 44, 2001-2007 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-11-2001

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited