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Applied Optics

Applied Optics


  • Vol. 44, Iss. 11 — Apr. 10, 2005
  • pp: 2013–2018

Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy

Derrick R. Chou, Bradley A. Bower, and Adam Wax  »View Author Affiliations

Applied Optics, Vol. 44, Issue 11, pp. 2013-2018 (2005)

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We present a low-cost, high-speed, retrofitted laser scanning module for microscopy. The cage-mounted system, with various available fiber-coupled sources, offers a real-time imaging alternative to costly commercial systems with capabilities for conventional or confocal reflectance and fluorescence applications as well as advanced laser scanning microscopy implementations. Reflectance images of a resolution target and confocal images of fluorescent polystyrene beads are presented for system characterization. Confocal fluorescence image stacks of T84 epithelial cancer cells are presented to demonstrate application to biological studies. This laser scanning module is a flexible, scalable, high-speed alternative to commercial laser scanning systems suitable for applications requiring a simple imaging tool and for teaching laboratories.

© 2005 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

Original Manuscript: July 6, 2004
Revised Manuscript: November 16, 2004
Manuscript Accepted: November 17, 2004
Published: April 10, 2005

Derrick R. Chou, Bradley A. Bower, and Adam Wax, "Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy," Appl. Opt. 44, 2013-2018 (2005)

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