OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 11 — Apr. 10, 2005
  • pp: 2013–2018

Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy

Derrick R. Chou, Bradley A. Bower, and Adam Wax  »View Author Affiliations


Applied Optics, Vol. 44, Issue 11, pp. 2013-2018 (2005)
http://dx.doi.org/10.1364/AO.44.002013


View Full Text Article

Enhanced HTML    Acrobat PDF (713 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a low-cost, high-speed, retrofitted laser scanning module for microscopy. The cage-mounted system, with various available fiber-coupled sources, offers a real-time imaging alternative to costly commercial systems with capabilities for conventional or confocal reflectance and fluorescence applications as well as advanced laser scanning microscopy implementations. Reflectance images of a resolution target and confocal images of fluorescent polystyrene beads are presented for system characterization. Confocal fluorescence image stacks of T84 epithelial cancer cells are presented to demonstrate application to biological studies. This laser scanning module is a flexible, scalable, high-speed alternative to commercial laser scanning systems suitable for applications requiring a simple imaging tool and for teaching laboratories.

© 2005 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

History
Original Manuscript: July 6, 2004
Revised Manuscript: November 16, 2004
Manuscript Accepted: November 17, 2004
Published: April 10, 2005

Citation
Derrick R. Chou, Bradley A. Bower, and Adam Wax, "Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy," Appl. Opt. 44, 2013-2018 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-11-2013

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited