OSA's Digital Library

Applied Optics

Applied Optics


  • Vol. 44, Iss. 14 — May. 10, 2005
  • pp: 2879–2886

Geometric determination of saturation in fluorescence spectroscopy

Michele Marrocco  »View Author Affiliations

Applied Optics, Vol. 44, Issue 14, pp. 2879-2886 (2005)

View Full Text Article

Enhanced HTML    Acrobat PDF (336 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



In laser spectroscopy, saturation of atomic or molecular transitions cannot be ignored, even at modest laser intensities. The saturation status is customarily diagnosed from measurements of saturation curves describing the dependence of spectroscopic signals on laser intensity. I propose an alternative method that relies on a geometric comparison of the spatial laser profile with images of the spectroscopic quantity under investigation. A single image can be used to determine the saturation status and its associated saturation laser intensity.

© 2005 Optical Society of America

OCIS Codes
(260.2510) Physical optics : Fluorescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(300.6410) Spectroscopy : Spectroscopy, multiphoton

Original Manuscript: February 25, 2004
Revised Manuscript: September 30, 2004
Manuscript Accepted: December 1, 2004
Published: May 10, 2005

Michele Marrocco, "Geometric determination of saturation in fluorescence spectroscopy," Appl. Opt. 44, 2879-2886 (2005)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. W. Demtröder, Laser Spectroscopy (Springer-Verlag, Berlin, 2003). [CrossRef]
  2. A. C. Eckbreth, Laser Diagnostics for Combustion Temperature and Species (Gordon & Breach, Amsterdam, 1996).
  3. J. W. Daily, “Saturation of fluorescence in flames with a Gaussian laser beam,” Appl. Opt. 17, 225–229 (1978). [CrossRef] [PubMed]
  4. J. W. Daily, “Laser-induced fluorescence spectroscopy in flames,” Prog. Energy Combust. Sci. 23, 133–199 (1997). [CrossRef]
  5. W. Denk, J. H. Strickler, W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248, 73–76 (1990). [CrossRef] [PubMed]
  6. C. Xu, W. W. Webb, “Measurements of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt. Soc. Am. B 13, 481–491 (1996). [CrossRef]
  7. M. Marrocco, “Spatial laser-wing suppression in saturated laser-induced fluorescence without spatial discrimination,” Opt. Lett. 28, 2016–2018 (2003). [CrossRef] [PubMed]
  8. R. L. Swofford, W. M. McClain, “The effect of spatial and temporal laser beam characteristics on two-photon absorption,” Chem. Phys. Lett. 34, 455–460 (1975). [CrossRef]
  9. T. Plakhotnik, D. Walser, M. Pirotta, A. Renn, U. P. Wild, “Nonlinear spectroscopy on a single quantum system: two-photon absorption of a single molecule,” Science 271, 1703–1705 (1996). [CrossRef]
  10. See the polylogarithmic function at http://mathworld.wolfram.com/Polylogarithm.html .
  11. S. Laporta, E. Remiddi, “The analytical value of the electron (g − 2) at order α3 in QED,” Phys. Lett. B 379, 283–291 (1996). [CrossRef]
  12. A. Marcano O., I. Urdaneta, “Fluorescence quantum yield of Rhodamine 101 in the presence of absorption saturation,” Appl. Phys. B 72, 207–213 (2001). [CrossRef]
  13. T. F. Johnston, R. H. Brady, W. Proffitt, “Powerful single-frequency ring dye laser spanning the visible spectrum,” Appl. Opt. 21, 2307–2316 (1982). [CrossRef] [PubMed]
  14. M. Marrocco, M. D'Apice, S. Giammartini, M. Magaldi, G. P. Romano, “Measurements of molecular carbon radical concentrations by saturated laser-induced fluorescence in hydrocarbon flames at atmospheric pressure,” in Laser Applications in Medicine, Biology, and Environmental Science, G. Mueller, V. V. Tuchin, G. G. Matvienko, C. Werner, V. Y. Panchenko, eds., Proc. SPIE5149, 187–196 (2002). [CrossRef]
  15. M. Marrocco, “An alternative approach to temporal laser-wing effects in saturated laser-induced fluorescence,” Appl. Phys. B 77, 65–70 (2003). [CrossRef]
  16. B. J. Kirby, R. K. Hanson, “CO2 imaging with saturated planar laser-induced vibrational fluorescence,” Appl. Opt. 40, 6136–6144 (2001). [CrossRef]
  17. See polylogarithm integral representations at http://functions.wolfram.com/ZetaFunctionsandPolylogarithms/PolyLog/07/01/01/ .

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited