A new surface plasmon resonance (SPR) imaging technique was proposed. After measurements were conducted at varying wavelengths, the wavelength affording the minimum brightness (SPR wavelength) was determined at each pixel of the image. A two-dimensional map of the SPR wavelength could be converted to a thickness profile by use of a nonlinear calibration curve, which was obtained by Fresnel calculation. An array of protein thin layers on a gold film was evaluated in air to present the layers’ surface structure in nanometer scale.
© 2005 Optical Society of America
Original Manuscript: August 13, 2004
Revised Manuscript: December 27, 2004
Manuscript Accepted: January 4, 2005
Published: June 10, 2005
Soichi Otsuki, Kaoru Tamada, and S. Wakida, "Wavelength-scanning surface plasmon resonance imaging," Appl. Opt. 44, 3468-3472 (2005)