We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent.
© 2005 Optical Society of America
Original Manuscript: October 5, 2004
Revised Manuscript: February 14, 2005
Manuscript Accepted: February 15, 2005
Published: July 1, 2005
Lluís Martínez-León, Giancarlo Pedrini, and Wolfgang Osten, "Applications of short-coherence digital holography in microscopy," Appl. Opt. 44, 3977-3984 (2005)