A novel fluorescence spectrometer and method for the simultaneous detection of multiple-fluorophore species in a no-moving-parts, instantaneous manner is described. In the reported embodiment of the instrument, a tapered Fabry–Perot filter is used to spatially encode the fluorescence spectrum from a multiple-dye-containing test sample. Using a pseudoinverse reconstruction algorithm, we spectrally decode the particle concentration for each dye specie in the test sample. Experimental results are reported along with a theoretical treatment of the method.
© 2005 Optical Society of America
Original Manuscript: November 10, 2004
Revised Manuscript: April 12, 2005
Manuscript Accepted: April 13, 2005
Published: September 1, 2005
Jeremy A. Wahl, Jay S. Van Delden, and Sandip Tiwari, "Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer," Appl. Opt. 44, 5190-5197 (2005)