Multiple-fluorophore-specie detection with a tapered Fabry-Perot fluorescence spectrometer
Applied Optics, Vol. 44, Issue 25, pp. 5190-5197 (2005)
http://dx.doi.org/10.1364/AO.44.005190
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Abstract
A novel fluorescence spectrometer and method for the simultaneous detection of multiple-fluorophore species in a no-moving-parts, instantaneous manner is described. In the reported embodiment of the instrument, a tapered Fabry-Perot filter is used to spatially encode the fluorescence spectrum from a multiple-dye-containing test sample. Using a pseudoinverse reconstruction algorithm, we spectrally decode the particle concentration for each dye specie in the test sample. Experimental results are reported along with a theoretical treatment of the method.
© 2005 Optical Society of America
OCIS Codes
(120.2230) Instrumentation, measurement, and metrology : Fabry-Perot
(120.2440) Instrumentation, measurement, and metrology : Filters
(260.3160) Physical optics : Interference
Citation
Jeremy A. Wahl, Jay S. Van Delden, and Sandip Tiwari, "Multiple-fluorophore-specie detection with a tapered Fabry-Perot fluorescence spectrometer," Appl. Opt. 44, 5190-5197 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-25-5190
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