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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 25 — Sep. 1, 2005
  • pp: 5190–5197

Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer

Jeremy A. Wahl, Jay S. Van Delden, and Sandip Tiwari  »View Author Affiliations


Applied Optics, Vol. 44, Issue 25, pp. 5190-5197 (2005)
http://dx.doi.org/10.1364/AO.44.005190


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Abstract

A novel fluorescence spectrometer and method for the simultaneous detection of multiple-fluorophore species in a no-moving-parts, instantaneous manner is described. In the reported embodiment of the instrument, a tapered Fabry–Perot filter is used to spatially encode the fluorescence spectrum from a multiple-dye-containing test sample. Using a pseudoinverse reconstruction algorithm, we spectrally decode the particle concentration for each dye specie in the test sample. Experimental results are reported along with a theoretical treatment of the method.

© 2005 Optical Society of America

OCIS Codes
(120.2230) Instrumentation, measurement, and metrology : Fabry-Perot
(120.2440) Instrumentation, measurement, and metrology : Filters
(260.3160) Physical optics : Interference

History
Original Manuscript: November 10, 2004
Revised Manuscript: April 12, 2005
Manuscript Accepted: April 13, 2005
Published: September 1, 2005

Citation
Jeremy A. Wahl, Jay S. Van Delden, and Sandip Tiwari, "Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer," Appl. Opt. 44, 5190-5197 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-25-5190


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