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Applied Optics

Applied Optics


  • Vol. 44, Iss. 25 — Sep. 1, 2005
  • pp: 5190–5197

Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer

Jeremy A. Wahl, Jay S. Van Delden, and Sandip Tiwari  »View Author Affiliations

Applied Optics, Vol. 44, Issue 25, pp. 5190-5197 (2005)

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A novel fluorescence spectrometer and method for the simultaneous detection of multiple-fluorophore species in a no-moving-parts, instantaneous manner is described. In the reported embodiment of the instrument, a tapered Fabry–Perot filter is used to spatially encode the fluorescence spectrum from a multiple-dye-containing test sample. Using a pseudoinverse reconstruction algorithm, we spectrally decode the particle concentration for each dye specie in the test sample. Experimental results are reported along with a theoretical treatment of the method.

© 2005 Optical Society of America

OCIS Codes
(120.2230) Instrumentation, measurement, and metrology : Fabry-Perot
(120.2440) Instrumentation, measurement, and metrology : Filters
(260.3160) Physical optics : Interference

Original Manuscript: November 10, 2004
Revised Manuscript: April 12, 2005
Manuscript Accepted: April 13, 2005
Published: September 1, 2005

Jeremy A. Wahl, Jay S. Van Delden, and Sandip Tiwari, "Multiple-fluorophore-specie detection with a tapered Fabry–Perot fluorescence spectrometer," Appl. Opt. 44, 5190-5197 (2005)

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  1. T. B. Hirschfeld, M. J. Block, “Fluorescent immunoassay using optical fibers and antibodies immobilized on surfaces,” presented at the Federation of Analytical Chemistry and Spectroscopy Societies Eleventh Annual Meeting, Philadelphia, Pa., 16 September 1984.
  2. M. J. Block, T. B. Hirschfeld, “Apparatus including optical fiber for fluorescence immunoassay,” U.S. patent4,582,809 (15April1986).
  3. E. W. Saaski, C. C. Jung, “Assay methods and apparatus,” U.S. patent6,136,611 (24October2000).
  4. C. C. Jung, E. W. Saaski, D. A. McCrae, B. M. Lingerfelt, G. P. Anderson, “RAPTOR: a fluoroimmunoassay-based fiber optic sensor for detection of biological threats,” IEEE Sensors J. 3, 352–360 (2003). [CrossRef]
  5. M. Gouzman, N. Lifshitz, S. Luryi, O. Semyonov, D. Gavrilov, V. Kuzminskiy, “Excitation-emission fluorimeter based on linear interference filters,” Appl. Opt. 43, 3066–3072 (2004). [CrossRef] [PubMed]
  6. J. A. Wahl, J. S. Van Delden, S. Tiwari, “Tapered Fabry–Perot filters,” IEEE Photon. Technol. Lett. 16, 1873–1875 (2004). [CrossRef]
  7. J. D. Gaskill, Linear Systems, Fourier Transforms, and Optics (Wiley, 1978).
  8. H. H. Barrett, K. J. Myers, Foundations of Image Science (Wiley, 2003).
  9. Y. Garini, A. Gil, I. Bar-Am, D. Cabib, N. Katzir, “Signal to noise analysis of multiple color fluorescence imaging microscopy,” Cytometry 35, 214–226 (1999). [CrossRef] [PubMed]
  10. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic, 1999). [CrossRef]

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