OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 25 — Sep. 1, 2005
  • pp: 5239–5248

Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging

David F. Wilson, Sergei A. Vinogradov, Pavel Grosul, M. Noel Vaccarezza, Akiko Kuroki, and Jean Bennett  »View Author Affiliations


Applied Optics, Vol. 44, Issue 25, pp. 5239-5248 (2005)
http://dx.doi.org/10.1364/AO.44.005239


View Full Text Article

Acrobat PDF (2415 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Maps of the oxygen distribution in the retina of the mouse eye were obtained by phosphorescence-lifetime imaging. Phosphor dissolved in the blood was excited by modulated light and phosphorescence imaged through microscope optics with an intensified-CCD camera. Phosphorescence lifetimes and oxygen pressures were calculated for each pixel of the images. The resolution was sufficient to permit the detection of anomalies that result in reduced oxygen pressures in individual retinal capillaries. High-resolution maps of oxygen distribution in the retina can provide greater understanding of the role of oxygen and vascular function in diseases of the eye.

© 2005 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

Citation
David F. Wilson, Sergei A. Vinogradov, Pavel Grosul, M. Noel Vaccarezza, Akiko Kuroki, and Jean Bennett, "Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging," Appl. Opt. 44, 5239-5248 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-25-5239

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited