OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 26 — Sep. 10, 2005
  • pp: 5468–5474

Effects of background fluorescence in fluorescence molecular tomography

Melisa Gao, George Lewis, Gordon M. Turner, Antoine Soubret, and Vasilis Ntziachristos  »View Author Affiliations


Applied Optics, Vol. 44, Issue 26, pp. 5468-5474 (2005)
http://dx.doi.org/10.1364/AO.44.005468


View Full Text Article

Enhanced HTML    Acrobat PDF (810 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Recent advances in optical imaging systems and systemically administered fluorescent probes have significantly improved the ways by which we can visualize proteomics in vivo. A key component in the design of fluorescent probes is a favorable biodistribution, i.e., localization only in the targeted diseased tissue, in order to achieve high contrast and good detection characteristics. In practice, however, there is always some level of background fluorescence present that could result in distorted or obscured visualization and quantification of measured signals. In this study we observe the effects of background fluorescence in tomographic imaging. We demonstrate that increasing levels of background fluorescence result in artifacts when using a linear perturbation algorithm, along with a significant loss of image fidelity and quantification accuracy. To correct for effects of background fluorescence, we have applied cubic polynomial fits to bulk raw measurements obtained from spatially homogeneous and heterogeneous phantoms. We show that subtraction of the average fluorescence response from the raw data before reconstruction can improve image quality and quantification accuracy as shown in relatively homogeneous or heterogeneous phantoms. Subtraction methods thus appear to be a promising route for adaptively correcting nonspecific background fluorochrome distribution.

© 2005 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.6960) Medical optics and biotechnology : Tomography
(260.2510) Physical optics : Fluorescence

History
Original Manuscript: November 11, 2004
Revised Manuscript: April 28, 2005
Manuscript Accepted: May 5, 2005
Published: September 10, 2005

Citation
Melisa Gao, George Lewis, Gordon M. Turner, Antoine Soubret, and Vasilis Ntziachristos, "Effects of background fluorescence in fluorescence molecular tomography," Appl. Opt. 44, 5468-5474 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-26-5468


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. V. Ntziachristos, C. Tung, C. Bremer, R. Weissleder, “Fluorescence-mediated tomography resolves protease activity in vivo,” Nat. Med. 8, 757–760 (2002). [CrossRef] [PubMed]
  2. M. A. Oleary, D. A. Boas, X. D. Li, B. Chance, A. G. Yodh, “Fluorescence lifetime imaging in turbid media,” Opt. Lett. 21, 158–160 (1996). [CrossRef]
  3. B. B. Das, F. Liu, R. R. Alfano, “Time-resolved fluorescence and photon migration studies in biomedical and model random media,” Rep. Prog. Phys. 60, 227–292 (1997). [CrossRef]
  4. M. J. Eppstein, D. E. Dougherty, T. L. Troy, E. M. Sevick-Muraca, “Biomedical optical tomography using dynamic parameterization and Bayesian conditioning on photon migration measurements,” Appl. Opt. 38, 2138–2150 (1999). [CrossRef]
  5. H. B. Jiang, “Frequency-domain fluorescent diffusion tomography: a finite-element-based algorithm and simulations,” Appl. Opt. 37, 5337–5343 (1998). [CrossRef]
  6. J. Chang, H. L. Graber, R. L. Barbour, “Imaging of fluorescence in highly scattering media,” IEEE Trans. Biomed. Eng. 44, 810–822 (1997). [CrossRef] [PubMed]
  7. S. R. Arridge, “Optical tomography in medical imaging,” Inverse Probl. 15, R41–R93 (1999). [CrossRef]
  8. R. Barbour, S. Blattman, T. Panetta, in Dynamic Optical Tomography: a New Approach for Investigating Tissue-Vascular Coupling in Large Tissue Structures, OSA Technical Digest (Optical Society of America, 2000), pp. 336–338.
  9. D. A. Boas, L. E. Campbell, A. G. Yodh, “Scattering and imaging with diffusing temporal field correlations,” Phys. Rev. Lett. 75, 1855–1858 (1995). [CrossRef] [PubMed]
  10. A. D. Klose, A. H. Hielscher, “Iterative reconstruction scheme for optical tomography based on the equation of radiative transfer,” Med. Phys. 26, 1698–1707 (1999). [CrossRef] [PubMed]
  11. M. O’Leary, D. Boas, B. Chance, A. Yodh, “Experimental images of heterogeneous turbid media by frequency-domain diffusing-photon tomography,” Opt. Lett. 20, 426–428 (1995). [CrossRef]
  12. V. Ntziachristos, R. Weissleder, “Experimental three-dimensional fluorescence reconstruction of diffuse media using a normalized Born approximation,” Opt. Lett. 26, 893–895 (2001). [CrossRef]
  13. A. Godavarty, A. B. Thompson, R. Roy, M. Gurfinkel, M. J. Eppstein, C. Zhang, E. M. Sevick-Muraca, “Diagnostic imaging of breast cancer using fluorescence-enhanced optical tomography: phantom studies,” J. Biomed. Opt. 9, 488–496 (2004). [CrossRef] [PubMed]
  14. E. Graves, J. Ripoll, R. Weissleder, V. Ntziachristos, “A sub-millimeter resolution fluorescence molecular imaging system for small animal imaging,” Med. Phys. 30, 901–911 (2003). [CrossRef] [PubMed]
  15. V. Ntziachristos, E. Schellenberger, J. Ripoll, D. Yessayan, E. Graves, A. Bogdanov, A. L. Josephson, R. Weissleder, “Visualization of anti-tumor treatment by means of fluorescence molecular tomography using an annexin V–Cy5.5 conjugate,” Proc. Natl. Acad. Sci. U.S.A. 101, 12294–12299 (2004). [CrossRef]
  16. H. R. Herschman, “Molecular imaging: looking at problems, seeing solutions,” Science 302, 605–608 (2003). [CrossRef] [PubMed]
  17. R. Weissleder, V. Ntziachristos, “Shedding light onto live molecular targets,” Nat. Med. 9, 123–128 (2003). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited