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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Glenn D. Boreman
  • Vol. 44, Iss. 28 — Oct. 1, 2005
  • pp: 5928–5936

Confocal microscopy using variable-focal-length microlenses and an optical fiber bundle

Lisong Yang, Aaron Mac Raighne, Eithne M. McCabe, L. Andrea Dunbar, and Toralf Scharf  »View Author Affiliations


Applied Optics, Vol. 44, Issue 28, pp. 5928-5936 (2005)
http://dx.doi.org/10.1364/AO.44.005928


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Abstract

The use of variable-focal-length (VFL) microlenses can provide a way to axially scan the foci across a sample by electronic control. We demonstrate an approach to coupling VFL microlenses individually to a fiber bundle as a way to create a high-throughput aperture array with a controllable aperture pattern. It would potentially be applied in real-time confocal imaging in vivo for biological specimens. The VFL microlenses that we used consist of a liquid-crystal film sandwiched between a pair of conductive substrates for which one has a hole-patterned electrode. One obtains the variation of the focal length by changing the applied voltage. The fiber bundle has been characterized by coupling with both coherent and incoherent light sources. We further demonstrate the use of a VFL microlens array in combination with the fiber bundle to build up a confocal system. The axial response of the confocal system has been measured without mechanical movement of the sample or the objective, and the FWHM is estimated to be approximately 16 µm, with asymmetric sidelobes.

© 2005 Optical Society of America

OCIS Codes
(060.0060) Fiber optics and optical communications : Fiber optics and optical communications
(060.2310) Fiber optics and optical communications : Fiber optics
(180.1790) Microscopy : Confocal microscopy
(350.3950) Other areas of optics : Micro-optics

ToC Category:
Microscopy

Citation
Lisong Yang, Aaron Mac Raighne, Eithne M. McCabe, L. Andrea Dunbar, and Toralf Scharf, "Confocal microscopy using variable-focal-length microlenses and an optical fiber bundle," Appl. Opt. 44, 5928-5936 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-28-5928


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References

  1. T. Wilson and C. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic, 1984).
  2. M. D. Egger and M. Petrán, "New reflected-light microscope for viewing unstained brain and ganglion cells," Science 157, 305-307 (1967).
  3. E. M. McCabe, D. T. Fewer, A. C. Ottewill, S. J. Hewlett, and J. Hegarty, "Direct-view microscopy: optical sectioning strength for finite sized, multiple-pinhole arrays," J. Microsc. 184, 95-105 (1996).
  4. T. Tanaami, Y. Sugiyama, and K. Mikuriya, "High speed confocal laser microscopy," Yokogawa Tech. Rep. 19, 7-10 (Yokogawa, 9-32 Nakacho 2-chome, Musashino-shi, Tokyo, Japan, 1994).
  5. A. F. Gmitro and D. Aziz, "Confocal microscopy through a fiber-optic imaging bundle," Opt. Lett. 18, 565-567 (1993).
  6. R. Juskaitis, T. Wilson, and T. F. Watson, "Real-time white light reflection confocal microscopy using a fibre-optic bundle," Scanning 19, 15-19 (1997).
  7. E. McCabe, "Optical imaging systems," Irish patent S99 0004 (4 April 2001).
  8. P. J. Smith, C. M. Taylor, E. M. McCabe, D. R. Selviah, S. E. Day, and L. G. Commander, "Switchable fiber coupling using variable-focal-length microlenses," Rev. Sci. Instrum. 72, 3132-3134 (2001). [CrossRef]
  9. D. W. Berreman, "Variable focus liquid crystal lens system," U.S. patent 4,190,330 (26 February, 1980).
  10. S. Sato, "Liquid crystal lens-cell with variable focal length," Jpn. J. Appl. Phy. 18, 1679-1684 (1979).
  11. T. Scharf, P. Kipfer, M. Bouvier, and J. Grupp, "Diffraction limited liquid crystal microlenses with planar alignment," Jpn. J. Appl. Phys. 39, 6629-6636 (2000). [CrossRef]
  12. S. Nose, S. Masuda, and S. Sato, "Optical properties of a liquid crystal microlens with a symmetric electrode structure," Jpn. J. Appl. Phys. 30, L2110-2112 (1991). [CrossRef]
  13. C. J. R. Sheppard, M. Gu, K. Brain, and H. Zhou, "Influence of spherical aberration on axial imaging of confocal reflection microscopy," Appl. Opt. 33, 616-624 (1994).

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