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Applied Optics

Applied Optics


  • Vol. 44, Iss. 3 — Jan. 20, 2005
  • pp: 297–304

Formulation and implementation of a phase-resolved fluorescence technique for latent-fingerprint imaging: theoretical and experimental analysis

U. S. Dinish, Z. X. Chao, L. K. Seah, A. Singh, and V. M. Murukeshan  »View Author Affiliations

Applied Optics, Vol. 44, Issue 3, pp. 297-304 (2005)

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A theoretical and experimental study of the imaging of latent fingerprints by a phase-resolved fluorescence technique along with associated signal-processing analysis is described. The system configuration is optimized by incorporation of a novel approach of homodyne-assisted even-step phase shifting in a signal-processing concept. The excitation laser source and gain of the detection device, which are modulated at megahertz frequency followed by sensitive signal-processing concepts, are employed to separate the fingerprint fluorescence from background fluorescence. Experiments are carried out with fingerprints deposited upon different types of substrate surfaces. Later, a quantitative image-quality assessment is carried out, which confirms the improvement in the quality of the phase-resolved fingerprint image. Imaging of older fingerprints with better contrast is also carried out with the proposed novel technique.

© 2005 Optical Society of America

OCIS Codes
(070.6020) Fourier optics and signal processing : Continuous optical signal processing
(110.3000) Imaging systems : Image quality assessment
(120.2920) Instrumentation, measurement, and metrology : Homodyning
(300.2530) Spectroscopy : Fluorescence, laser-induced

Original Manuscript: May 17, 2004
Revised Manuscript: September 30, 2004
Manuscript Accepted: October 7, 2004
Published: January 20, 2005

U. S. Dinish, Z. X. Chao, L. K. Seah, A. Singh, and V. M. Murukeshan, "Formulation and implementation of a phase-resolved fluorescence technique for latent-fingerprint imaging: theoretical and experimental analysis," Appl. Opt. 44, 297-304 (2005)

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  1. P. Margot, C. Lennard, Fingerprint Detection Techniques (Institut de Police Scientifique et de Criminologie, Université de Lausanne, Lausanne, Switzerland, 1994).
  2. R. Saferstein, Criminalistics: An Introduction to Forensic Science (Prentice-Hall, Englewood Cliffs, N.J., 1981).
  3. J. F. Cowger, Friction Ridge Skin: Comparison and Identification of Fingerprints (Elsevier, New York, 1983).
  4. B. E. Dalrymple, J. M. Duff, E. R. Menzel, “Inherent fingerprint luminescence—detection by laser,” J. Forensic Sci. 22, 106–115 (1977).
  5. E. R. Menzel, “Recent advances in photoluminescence detection of fingerprints,” Sci. World J. 1, 498–509 (2001). [CrossRef]
  6. R. H. Murdock, E. R. Menzel, “A computer interfaced time-resolved luminescence imaging system,” J. Forensic Sci. 38, 521–529 (1993).
  7. L. K. Seah, U. S. Dinish, S. K. Ong, Z. X. Chao, V. M. Murukeshan, “Time-resolved imaging of latent fingerprints with nanosecond resolution,” Opt. Laser Technol. 36, 371–376 (2004). [CrossRef]
  8. L. B. McGown, D. S. Kreiss, “Spectral fingerprinting using phase-resolved fluorescence spectroscopy,” in Fluorescence Detection II, E. R. Menzel, ed., Proc. SPIE910, 73–79 (1988). [CrossRef]
  9. U. S. Dinish, L. K. Seah, V. M. Murukeshan, L. S. Ong, “Theoretical analysis of phase-resolved fluorescence emission from fingerprint samples,” Opt. Commun. 223, 55–60 (2003). [CrossRef]
  10. J. A. Steinkamp, H. A. Crissman, “Resolution of heterogenous fluorescence emission signals and decay lifetime measurement on fluorochrome-labelled cells by phase sensitive FCM,” in Advances in Fluorescence Sensing Technology, J. R. Lakowicz, R. B. Thompson, eds., Proc. SPIE1885, 278–289 (1993). [CrossRef]
  11. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic, New York, 1999). [CrossRef]
  12. Z. X. Chao, U. S. Dinish, L. K. Seah, V. M. Murukeshan, “Homodyne and heterodyne signal processing assisted phase resolved optical technique for latent fingerprint imaging: a theoretical study,” J. Mod. Opt. (to be published).
  13. D. Erimberger, D. Zaak, H. Stepp, R. Knuchel, R. Baumgartner, P. Schneede, N. Schmeller, A. Hofstetter, “Autofluorescence imaging to optimise 5-ALA- induced fluorescence endoscopy of bladder carcinoma,” Urology 58, 372–375 (2001). [CrossRef]
  14. M. Zargi, I. Fajdiga, L. Smid, “Autofluorescence imaging in the diagnosis of laryngeal cancer,” Eur. Arch. Otolaryng. Rhino-L 257, 17–23 (2000). [CrossRef]
  15. L. K. Seah, V. M. Murukeshan, L. S. Ong, Z. X. Chao, U. S. Dinish, S. K. Ong, “Method and apparatus for imaging latent fingerprints,” Singapore patent file number 200307736-9 (29December2003).

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