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Applied Optics

Applied Optics


  • Editor: Glenn D. Boreman
  • Vol. 44, Iss. 30 — Oct. 20, 2005
  • pp: 6486–6495

Principal-components analysis of fluorescence cross-section spectra from pathogenic and simulant bacteria

Harold I. Heaton  »View Author Affiliations

Applied Optics, Vol. 44, Issue 30, pp. 6486-6495 (2005)

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Principal-components analysis of a new set of highly resolved (<1 nm) fluorescence cross-section spectra excited at 354.7 nm over the 370–646 nm band has been used to demonstrate the potential ability of UV standoff lidars to discriminate among particular biological warfare agents and simulants over short ranges. The remapped spectra produced by this technique from Bacillus globigii (Bg) and Bacillus anthracis (Ba) spores were sufficiently different to allow them to be cleanly separated, and the Ba spectra obtained from Sterne and Ames strain spores were distinguishable. These patterns persisted as the spectral resolution was subsequently degraded in processing from ~1 to 34 nm. This is to the author’s knowledge the first time that resolved fluorescence spectra from biological warfare agents have been speciated or shown to be distinguishably different from those normally used surrogates by optical spectroscopy.

© 2005 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(000.5490) General : Probability theory, stochastic processes, and statistics
(170.1580) Medical optics and biotechnology : Chemometrics
(280.3640) Remote sensing and sensors : Lidar
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:

Original Manuscript: January 10, 2005
Revised Manuscript: June 1, 2005
Manuscript Accepted: June 17, 2005
Published: October 20, 2005

Harold I. Heaton, "Principal-components analysis of fluorescence cross-section spectra from pathogenic and simulant bacteria," Appl. Opt. 44, 6486-6495 (2005)

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