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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 44, Iss. 7 — Mar. 1, 2005
  • pp: 1160–1170

Detection of fecal contamination on apples with nanosecond-scale time-resolved imaging of laser-induced fluorescence

Alan M. Lefcourt, Moon S. Kim, and Yud-Ren Chen  »View Author Affiliations


Applied Optics, Vol. 44, Issue 7, pp. 1160-1170 (2005)
http://dx.doi.org/10.1364/AO.44.001160


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Abstract

Detection of apples contaminated with feces is a public health concern. We found that time-resolved imaging of apples artificially contaminated with feces allowed optimization of timing parameters for detection. Dairy feces were applied to Red Delicious and Golden Delicious apples. Laser-induced fluorescence responses were imaged by use of a gated intensified camera. We developed algorithms to automatically detect contamination iteratively by using one half of the apples and validated them by applying the optimized algorithms to the remaining apples. Results show that consideration of the timing of fluorescence responses to pulsed-laser excitation can enhance detection of feces on apples.

© 2005 Optical Society of America

OCIS Codes
(150.3040) Machine vision : Industrial inspection
(170.0110) Medical optics and biotechnology : Imaging systems
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

History
Original Manuscript: December 31, 2003
Revised Manuscript: May 19, 2004
Manuscript Accepted: October 25, 2004
Published: March 1, 2005

Citation
Alan M. Lefcourt, Moon S. Kim, and Yud-Ren Chen, "Detection of fecal contamination on apples with nanosecond-scale time-resolved imaging of laser-induced fluorescence," Appl. Opt. 44, 1160-1170 (2005)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-44-7-1160

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