Harmonic phase-dispersion microscopy (PDM) is a new imaging technique in which contrast is provided by differences in refractive index at two harmonically related wavelengths. We report a new configuration of the harmonic phase-dispersion microscope in a Mach–Zehnder geometry as an instrument for imaging biological samples. Several improvements on the earlier design are demonstrated, including a single-pass configuration and acousto-optic modulators for generating the heterodyne signals without mechanical arm scanning. We demonstrate quantitative phase-dispersion images of test structures and biological samples.
© 2005 Optical Society of America
Original Manuscript: June 3, 2004
Revised Manuscript: October 20, 2004
Manuscript Accepted: October 20, 2004
Published: March 1, 2005
Andrew Ahn, Changhuei Yang, Adam Wax, Gabriel Popescu, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Harmonic phase-dispersion microscope with a Mach–Zehnder interferometer," Appl. Opt. 44, 1188-1190 (2005)