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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 13 — May. 1, 2006
  • pp: 3049–3062

Task-based imaging of colon cancer in the ApcMin∕+ mouse model

James B. McNally, Nathaniel D. Kirkpatrick, Lida P. Hariri, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Urs Utzinger, and Jennifer K. Barton  »View Author Affiliations

Applied Optics, Vol. 45, Issue 13, pp. 3049-3062 (2006)

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Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The A p c M i n / + genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680   nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680   nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.

© 2006 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:

Original Manuscript: May 16, 2005
Revised Manuscript: December 4, 2005
Manuscript Accepted: December 9, 2005

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

James B. McNally, Nathaniel D. Kirkpatrick, Lida P. Hariri, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Urs Utzinger, and Jennifer K. Barton, "Task-based imaging of colon cancer in the ApcMin/+ mouse model," Appl. Opt. 45, 3049-3062 (2006)

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