Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Effect of washing on identification of Bacillus spores by principal-component analysis of fluorescence data

Not Accessible

Your library or personal account may give you access

Abstract

The fluorescence spectra of Bacillus spores are measured at excitation wavelengths of 280, 310, 340, 370, and 400  nm. When cluster analysis is used with the principal-component analysis, the Bacillus globigii spores can be distinguished from the other species of Bacillus spores (B. cereus, B. popilliae, and B. thuringiensis). To test how robust the identification process is with the fluorescence spectra, the B. globigii is obtained from three separate preparations in different laboratories. Furthermore the fluorescence is measured before and after washing and redrying the B. globigii spores. Using the cluster analysis of the first two or three principal components of the fluorescence spectra, one is able to distinguish B. globigii spores from the other species, independent of preparing or washing the spores.

© 2006 Optical Society of America

Full Article  |  PDF Article
More Like This
Fluorescence quantum efficiency of dry Bacillus globigii spores

Joseph Kunnil, Sivananthan Sarasanandarajah, Easaw Chacko, and Lou Reinisch
Opt. Express 13(22) 8969-8979 (2005)

Principal component analysis of fluorescence changes upon growth conditions and washing of Pseudomonas aeruginosa

Ekta Jhala, Craig Galilee, and Lou Reinisch
Appl. Opt. 46(22) 5522-5528 (2007)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved