OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 45, Iss. 16 — Jun. 1, 2006
  • pp: 3902–3912

Determination of scattering volume fraction and particle size distribution in the superficial layer of a turbid medium by using diffuse reflectance spectroscopy

Yasser S. Fawzy and Haishan Zeng  »View Author Affiliations


Applied Optics, Vol. 45, Issue 16, pp. 3902-3912 (2006)
http://dx.doi.org/10.1364/AO.45.003902


View Full Text Article

Enhanced HTML    Acrobat PDF (1050 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We have investigated the possibility of determining changes in the volume fraction of microstructure scatterers in the superficial tissue layers by using diffuse reflectance spectroscopy. To that extent we have built a two-layer optical phantom by using microparticles with various sizes in order to simulate the scattering properties of tissue microstructures. Reflectance spectral measurements were performed on a number of optical phantoms having different volume fractions of various microparticle sizes. An analytical model was developed using light-transport theory and fractal modeling approaches and was then fitted to the measured reflectance to calculate the volume fractions of the microparticles in phantoms. The results showed that we could measure changes in both the total volume fraction of the microparticles and in the overall size distribution of the microparticles with good accuracy ( > 80 % ) . These results suggest the potential of using this method for measuring the volume fraction changes of tissue microstructure scatterers and applications in the detection of cancerous related morphological and structural changes.

© 2006 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.0290) Scattering : Scattering
(290.5850) Scattering : Scattering, particles
(290.7050) Scattering : Turbid media

History
Original Manuscript: April 14, 2005
Revised Manuscript: August 16, 2005
Manuscript Accepted: November 3, 2005

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Yasser S. Fawzy and Haishan Zeng, "Determination of scattering volume fraction and particle size distribution in the superficial layer of a turbid medium by using diffuse reflectance spectroscopy," Appl. Opt. 45, 3902-3912 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-16-3902


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Sciler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, "Observation of periodic fine structure in reflectance from biological tissue: a new technique for measuring nuclear size distribution," Phys. Rev. Lett. 80, 627-630 (1998). [CrossRef]
  2. J. R. Mourant, A. H. Hielscher, A. A. Eick, T. M. Johnson, and J. P. Freyer, "Evidence of intrinsic differences in the light scattering properties of tumorigenic and nontumorigenic cells," Cancer 84, 366-374 (1998). [CrossRef]
  3. M. G. Muller, T. A. Valdez, I. Georgakoudi, V. Backman, C. Fuentes, S. Kabani, N. Laver, Z. Wang, C. W. Boone, R. R. Dasari, S. M. Shapshay, and M. S. Feld, "Spectroscopic detection and evaluation of morphologic and biochemical changes in early human oral carcinoma," Cancer 97, 1681-1692 (1997). [CrossRef]
  4. G. Zonios, L. T. Perelman, V. Backman, R. Manoharan, M. Fitzmaurice, J. Van Dam, and M. S. Feld, "Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo," Appl. Opt. 38, 6628-6637 (1999). [CrossRef]
  5. V. Backman, R. Gurjar, K. Badizadegan, I. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, "Polarized light scattering spectroscopy for quantitative measurements of epithelial cellular structures in situ," IEEE Trans. Biomed. Eng. 5, 1019-1026 (1999).
  6. J. R. Mourant, J. P. Freyer, A. H. Hielscher, A. A. Eick, D. Shen, and T. M. Jonson, "Mechanism of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics," Appl. Opt. 37, 3586-3593 (1998) [CrossRef]
  7. K. Sokolov, R. Drezek, K. Gossage, and R. Richards, "Reflectance spectroscopy with polarized light: is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). [CrossRef] [PubMed]
  8. J. M. Schmitt and G. Kumar, "Optical scattering properties of soft tissue: a discrete particle model," Appl. Opt. 37, 2788-2797 (1998). [CrossRef]
  9. V. Venugopalan, J. S. You, and B. J. Tromberg, "Radiative transport in the diffusion approximation: an extension for highly absorbing media and small source-detector separation," Phys. Rev. E 58, 2395-2407 (1998). [CrossRef]
  10. W. M. Star, in Optical-Thermal Response of Laser-Irradiated Tissue, A.J.Welch and M.J. C.van Gemert eds. (Plenum, 1995), pp. 131-206.
  11. C. F. Bohren and D. R. Huffman, Absorption and scattering of light by small particles (Wiley, 1983).
  12. C. Derman, L. J. Gleser, and I. Olkin, A Guide to Probability Theory and Application (Holt, Rinehart & Winston, 1973), Chap. 8, p. 378.
  13. B. Gelebart, E. Tinet, J. M. Tualle, and S. Avrillier, "Phase function simulation in tissue phantoms: a fractal approach," Pure Appl. Opt. 5, 377-388 (1996). [CrossRef]
  14. J. M. Schmitt and G. Kumar, "Turbulent nature of refractive-index variations in biological tissue," Opt. Lett. 21, 1310-1312 (1996). [CrossRef] [PubMed]
  15. T. H. Pham, T. Spot, L. O. Svaasand, and B. J. Tromberg, "Quantifying the properties of two-layer turbid media with frequency-domain diffuse reflectance," Appl. Opt. 39, 4733-4745 (2000). [CrossRef]
  16. W. H. Press, S. A. Teukolsky, W. T. Vetterling, and B. P. Flannery, Numerical Recipes in C, 2nd ed. (Cambridge U. Press, 1992).
  17. L.-H. Wang, S. L. Jacques, and L.-Q. Zheng, "MCML--Monte Carlo modeling of—hoton transport in multi-layered tissues" Comput. Methods Programs Biomed. 47, 131-146 (1995). [CrossRef] [PubMed]
  18. H. Zeng, M. Petek, M. T. Zorman, A. McWilliams, B. Paclic, and S. Lam, "Integrated endoscopy system for simultaneous imaging and spectroscopy for early lung cancer detection," Opt. Lett. 29, 587-589 (2004). [CrossRef] [PubMed]
  19. D. E. Hyde, T. J. Farrell, M. S. Patterson, and B. C. Wilson, "A diffusion theory model of spatially resolved fluorescence from depth-dependent fluorophore concentrations," Phys. Med. Biol. 46, 369-383 (2001). [CrossRef] [PubMed]
  20. E. Marx and G. W. Mulholland, "Size and refractive index determination of single polystyrene spheres," J. Res. Natl. Bur. Stand. 88, 321-338 (1983).
  21. J. R. Mourant, T. Fuselier, J. Boyer, T. M. Johnson, and I. J. Bigio, "Predictions and measurements of scattering and absorption over broad wavelength range in the tissue phantoms," Appl. Opt. 36, 949-957 (1997). [CrossRef] [PubMed]
  22. J. Qu, C. MacAulay, S. Lam, and B. Palcic, "Optical properties of normal and carcinomatous bronchial tissue," Appl. Opt. 31, 7397-7405 (1994). [CrossRef]
  23. J. R. Mourant, A. H. Hielscher, A. A. Eick, T. M. Johnson, and J. P. Freyer, "Evidence of intrinsic differences in the light scattering properties of tumorigenic and nontumorigenic cells," Cancer Cytopathol. 84, 336-374 (1998).
  24. H. Jiang, G. Marquez, and L. Wang, "Particle sizing in concentrated suspensions using steady-state, continuous-wave photon migration techniques," Opt. Lett. 23, 394-396 (1998). [CrossRef]
  25. M. Bartlett and H. Jiang, "Measurement of particle size-distribution in concentrated, rapidly flowing potassium chloride (KCl) suspensions using continuous-wave photon migration techniques," AIChE J. 47, 60-65 (2001). [CrossRef]
  26. R. C. Haskell, L. O. Svaasand, T. Tsay, T. C. Feng, M. S. McAdams, and B. J. Tomberg, "Boundary conditions for the diffusion equation in radiative transfer," J. Opt. Soc. Am. A 11, 2727-2743 (1994). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited