Confined diffusion in tubular structures analyzed by fluorescence correlation spectroscopy on a mirror
Applied Optics, Vol. 45, Issue 18, pp. 4497-4507 (2006)
http://dx.doi.org/10.1364/AO.45.004497
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Abstract
In fluorescence correlation spectroscopy (FCS) analysis it is generally assumed
that molecular species diffuse freely in volumes much larger than the three-dimensional
FCS observation volume. However, this standard assumption
is not valid in many measurement conditions, particularly in tubular
structures with diameters in the micrometer range, such as those found
in living cells (organelles, dendrites) and microfluidic devices (capillaries,
reaction chambers). As a result the measured autocorrelation functions
(ACFs) deviate from those predicted for free diffusion, and this can shift the
measured diffusion coefficient by as much as
© 2006 Optical Society of America
OCIS Codes
(120.2650) Instrumentation, measurement, and metrology : Fringe analysis
(120.3930) Instrumentation, measurement, and metrology : Metrological instrumentation
(170.1420) Medical optics and biotechnology : Biology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
ToC Category:
Spectroscopy
History
Original Manuscript: October 19, 2005
Revised Manuscript: January 12, 2006
Manuscript Accepted: January 18, 2006
Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics
Citation
Emilien Etienne, Pierre-François Lenne, James N. Sturgis, and Hervé Rigneault, "Confined diffusion in tubular structures analyzed by fluorescence correlation spectroscopy on a mirror," Appl. Opt. 45, 4497-4507 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-18-4497
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