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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 25 — Sep. 1, 2006
  • pp: 6634–6639

Emission wavelength dependence of fluorescence lifetimes of bacteriological spores and pollens

Ann Thomas, David Sands, Dave Baum, Leleng To, and Glenn O. Rubel  »View Author Affiliations

Applied Optics, Vol. 45, Issue 25, pp. 6634-6639 (2006)

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Concern about biological terrorism has greatly increased in the 21st century, and correspondingly, so has the need for accurate detection and identification of biological hazards, such as Bacillus anthracis. Optical techniques have been shown to be useful for this purpose. Use of fluorescence lifetimes as a function of emission wavelength for different materials using point- detection methods appears to be an additional viable option. Although the lifetimes range only between 2 and 6   ns , most biological materials tested in this study were distinguishable. A preliminary database has been compiled for use in a possible future detection system.

© 2006 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6500) Spectroscopy : Spectroscopy, time-resolved

Original Manuscript: September 16, 2005
Revised Manuscript: March 23, 2006
Manuscript Accepted: April 13, 2006

Virtual Issues
Vol. 1, Iss. 10 Virtual Journal for Biomedical Optics

Ann Thomas, David Sands, Dave Baum, Leleng To, and Glenn O. Rubel, "Emission wavelength dependence of fluorescence lifetimes of bacteriological spores and pollens," Appl. Opt. 45, 6634-6639 (2006)

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