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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 28 — Oct. 1, 2006
  • pp: 7309–7315

Interferometric tracking of optically trapped probes behind structured surfaces: a phase correction method

Peter C. Seitz, Ernst H. K. Stelzer, and Alexander Rohrbach  »View Author Affiliations

Applied Optics, Vol. 45, Issue 28, pp. 7309-7315 (2006)

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We investigate the influence of an additional scatterer on the tracking signal of an optically trapped particle. The three-dimensional particle position is recorded interferometrically with nanometer precision by using a quadrant photodiode in the back focal plane of a detection lens. A phase disturbance underneath the sample leads to incorrect position signals. The resulting interaction potential and forces are therefore erroneous as well. We present a procedure to correct for the disturbance by measuring its interferometric signal. We prove the applicability of our phase correction approach by generating a defined displacement of the trapped probe.

© 2006 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(140.7010) Lasers and laser optics : Laser trapping
(180.3170) Microscopy : Interference microscopy
(290.0290) Scattering : Scattering
(290.5850) Scattering : Scattering, particles

ToC Category:

Original Manuscript: February 23, 2006
Manuscript Accepted: April 20, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Peter C. Seitz, Ernst H. K. Stelzer, and Alexander Rohrbach, "Interferometric tracking of optically trapped probes behind structured surfaces: a phase correction method," Appl. Opt. 45, 7309-7315 (2006)

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  1. G. Binnig, C. F. Quate, and C. Gerber, "Atomic force microscope," Phys. Rev. Lett. 56, 930-933 (1986). [CrossRef] [PubMed]
  2. B.-W. Yang and H.-P. D. Shieh, "Interlayer cross talk in dual-layer read-only optical disks," Appl. Opt. 38, 333-338 (1999). [CrossRef]
  3. F. Roddier, ed., Adaptive Optics in Astronomy (Cambridge U. Press, 2004).
  4. M. Feierabend, M. Rückel, and W. Denk, "Coherence-gated wave-front sensing in strongly scattering samples," Opt. Lett. 29, 2255-2257 (2004). [CrossRef] [PubMed]
  5. C. J. R. Sheppard, M. Roy, and M. D. Sharma, "Image formation in low-coherence and confocal interference microscopes," Appl. Opt. 43, 1493-502 (2004). [CrossRef] [PubMed]
  6. M. G. L. Gustafsson, "Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution," Proc. Natl. Acad. Sci. U.S.A. 102, 13,081-13,086 (2005). [CrossRef]
  7. C. Bustamante, Z. Bryant, and S. B. Smith, "Ten years of tension: single-molecule DNA mechanics," Nature 421, 423-427 (2003). [CrossRef] [PubMed]
  8. M. W. Allersma, F. Gittes, M. J. deCastro, R. J. Stewart, and C. F. Schmidt, "Two-dimensional tracking of ncd motility by back focal plane interferometry," Biophys. J. 74, 1074-1085 (1998). [CrossRef] [PubMed]
  9. L. P. Ghislain and W. W. Webb, "Scanning-force microscope based on an optical trap," Opt. Lett. 18, 1678-1680 (1993). [CrossRef] [PubMed]
  10. H. Hertz, L. Malmqvist, L. Rosengren, and K. Ljungberg, "Optically trapped nonlinear particles as probes for scanning near-field optical microscopy," Ultramicroscopy 57, 309-312 (1995). [CrossRef]
  11. S. Kawata, Y. Inouye, and T. Sugiura, "Near-field scanning optical microscope with a laser trapped probe," Jpn. J. Appl. Phys. 33, L1725-L1727 (1994). [CrossRef]
  12. E. L. Florin, A. Pralle, J. K. Hörber, and E. H. Stelzer, "Photonic force microscope based on optical tweezers and two-photon excitation for biological applications," J. Struct. Biol. 119, 202-11 (1997). [CrossRef] [PubMed]
  13. G. M. Whitesides, J. K. Kriebel, and J. C. Love, "Molecular engineering of surfaces using self-assembled monolayers," Sci. Prog. 88, 17-48 (2005). [CrossRef] [PubMed]
  14. J. Groll, K. Albrecht, P. Gasteier, S. Riethmueller, U. Ziener, and M. Moeller, "Nanostructured ordering of fluorescent markers and single proteins on substrates," ChemBioChem 6, 1782-7 (2005). [CrossRef] [PubMed]
  15. H. Kress, E. H. K. Stelzer, G. Griffiths, and A. Rohrbach, "Control of relative radiation pressure in optical traps: application to phagocytic membrane binding studies," Phys. Rev. E 71, 061927 (2005). [CrossRef]
  16. A. Pralle, P. Keller, E. L. Florin, K. Simons, and J. K. Hörber, "Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells," J. Cell Biol. 148, 997-1008 (2000). [CrossRef] [PubMed]
  17. N. B. Becker, S. M. Altmann, T. Scholz, J. K. H. Hörber, E. H. K. Stelzer, and A. Rohrbach, "Three-dimensional bead position histograms reveal single-molecule nanomechanics," Phys. Rev. E 71, 021907 (2005).
  18. A. Rohrbach, C. Tischer, D. Neumayer, E.-L. Florin, and E. H. K. Stelzer, "Trapping and tracking a local probe with a photonic force microscope," Rev. Sci. Instrum. 75, 2197-2210 (2004). [CrossRef]
  19. A. Pralle, M. Prummer, E. L. Florin, E. H. Stelzer, and J. K. Hörber, "Three- dimensional high-resolution particle tracking for optical tweezers by forward scattered light," Microsc. Res. Tech. 44, 378-86 (1999). [CrossRef] [PubMed]
  20. A. Rohrbach, H. Kress, and E. H. K. Stelzer, "Three-dimensional tracking of small spheres in focused laser beams: influence of the detection angular aperture," Opt. Lett. 28, 411-413 (2003). [CrossRef] [PubMed]
  21. A. Jonás, P. Zemánek, and E.-L. Florin, "Single-beam trapping in front of reflective surfaces," Opt. Lett. 26, 1466-1468 (2001). [CrossRef]
  22. K. C. Neuman and S. M. Block, "Optical trapping," Rev. Sci. Instrum. 75, 2787-2809 (2004). [CrossRef]
  23. A. Rohrbach and E. H. K. Stelzer, "Optical trapping of dielectric particles in arbitrary fields," J. Opt. Soc. Am. A 18, 839-853 (2001). [CrossRef]

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