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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 45, Iss. 28 — Oct. 1, 2006
  • pp: 7529–7537

Simultaneous extraction of optical transport parameters and intrinsic fluorescence of tissue mimicking model media using a spatially resolved fluorescence technique

Sharad Gupta, V. L. N. Sridhar Raja, and Asima Pradhan  »View Author Affiliations


Applied Optics, Vol. 45, Issue 28, pp. 7529-7537 (2006)
http://dx.doi.org/10.1364/AO.45.007529


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Abstract

We present a method based on spatially resolved fluorescence measurement for the simultaneous estimation of optical transport parameters, namely, the reduced scattering coefficient ( μ s ) , the absorption coefficient ( μ a ) , and the intrinsic fluorescence spectra from turbid media. The accuracy of this approach was tested by conducting studies on a series of tissue-simulating phantoms with known optical transport properties. The estimated relative error in the values for μ s and μ a using this technique was found to be 10 % . Furthermore, the line shape and intensity of the intrinsic fluorescence recovered by using this approach were observed to be free from the distorting effects of the wavelength-dependent absorption and scattering properties of the medium, and they were in excellent agreement with the directly measured intrinsic fluorescence spectra of the fluorophores.

© 2006 Optical Society of America

OCIS Codes
(160.4760) Materials : Optical properties
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.7050) Medical optics and biotechnology : Turbid media
(290.1990) Scattering : Diffusion
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 4, 2005
Manuscript Accepted: April 25, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Sharad Gupta, V. L. N. Sridhar Raja, and Asima Pradhan, "Simultaneous extraction of optical transport parameters and intrinsic fluorescence of tissue mimicking model media using a spatially resolved fluorescence technique," Appl. Opt. 45, 7529-7537 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-28-7529

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