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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 45, Iss. 3 — Jan. 20, 2006
  • pp: 445–450

Proteins and dipicolinic acid released during heat shock activation of Bacillus subtilis spores probed by optical spectroscopy

Alexandra Alimova, A. Katz, Paul Gottlieb, and R. R. Alfano  »View Author Affiliations


Applied Optics, Vol. 45, Issue 3, pp. 445-450 (2006)
http://dx.doi.org/10.1364/AO.45.000445


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Abstract

UV fluorescence and absorption spectroscopy from Bacillus subtilis spores detected proteins and dipicolinic acid (DPA) released into the supernatant after heat treatments ranging from 20° to 90 ° C . The protein and DPA concentration in the supernatant was greater with higher heat treatment temperatures, undergoing a substantial increase for T 6 0 ° C , and supporting the theory that spores undergo a phase transition from a glassylike to a rubberylike state at 56 ° C . Gel electrophoresis detected several small proteins with molecular weights between 6   and   11   kDa . These proteins may be small acid-soluble spore proteins that are present in spores but break down during germination. A 30 kDa protein extracted above 60 ° C is related to the rubber–glass phase transition.

© 2006 Optical Society of America

OCIS Codes
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Virtual Issues
Vol. 1, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Alexandra Alimova, A. Katz, Paul Gottlieb, and R. R. Alfano, "Proteins and dipicolinic acid released during heat shock activation of Bacillus subtilis spores probed by optical spectroscopy," Appl. Opt. 45, 445-450 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-3-445

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