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Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 31 — Nov. 1, 2006
  • pp: 7977–7986

Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields

Jing Zhang, Mark C. Pitter, Shugang Liu, Chungwah See, and Mike G. Somekh  »View Author Affiliations

Applied Optics, Vol. 45, Issue 31, pp. 7977-7986 (2006)

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We report bright-field and dark-field surface-plasmon imaging using a modified solid immersion lens and a commercial objective of moderate NA in the epi configuration. The contrast and resolution are extremely good, giving well-resolved images of protein monolayers both in air and in water. We also describe a two-part solid immersion lens that allows the sample to be moved without degrading the image quality in any observable way. The merits of the two-part lens are discussed and compared to commercially available microscope objectives. Finally, we introduce a simple Green's function model that illustrates the key features of both bright-field and dark-field surface-plasmon imaging.

© 2006 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(110.0180) Imaging systems : Microscopy
(240.6680) Optics at surfaces : Surface plasmons

Original Manuscript: March 22, 2006
Manuscript Accepted: May 18, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Jing Zhang, Mark C. Pitter, Shugang Liu, Chungwah See, and Mike G. Somekh, "Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields," Appl. Opt. 45, 7977-7986 (2006)

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