OSA's Digital Library

Applied Optics

Applied Optics


  • Editor: James C. Wyant
  • Vol. 45, Iss. 32 — Nov. 10, 2006
  • pp: 8366–8373

Hyperspectral diffuse reflectance imaging for rapid, noncontact measurement of the optical properties of turbid materials

Jianwei Qin and Renfu Lu  »View Author Affiliations

Applied Optics, Vol. 45, Issue 32, pp. 8366-8373 (2006)

View Full Text Article

Enhanced HTML    Acrobat PDF (669 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a method and technique of using hyperspectral diffuse reflectance for rapid determination of the optical properties of turbid media. A hyperspectral imaging system in line scanning mode was used to acquire spatial diffuse reflectance profiles from liquid phantoms made up of absorbing dyes and fat emulsion scatterers over the spectral range of 450 1000 nm instantaneously. The hyperspectral reflectance data were analyzed by using a steady-state diffusion approximation model for semi-infinite homogeneous media. A calibration procedure was developed to compensate the nonuniform instrument response of the imaging system, and a curve-fitting algorithm was used to extract absorption and reduced scattering coefficients ( μ a and μ s , respectively) for the phantoms in the wavelength range from 530 to 900 nm . The hyperspectral imaging system gave good measures of μ a and μ s for the phantoms with average fitting errors of 12 % and 7 % , respectively. The hyperspectral imaging technique is fast, noncontact, and easy to use, which makes it especially suitable for measurement of the optical properties of turbid liquid and solid foods.

© 2006 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.7050) Medical optics and biotechnology : Turbid media
(290.0290) Scattering : Scattering
(300.1030) Spectroscopy : Absorption

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 16, 2006
Revised Manuscript: May 18, 2006
Manuscript Accepted: August 4, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Jianwei Qin and Renfu Lu, "Hyperspectral diffuse reflectance imaging for rapid, noncontact measurement of the optical properties of turbid materials," Appl. Opt. 45, 8366-8373 (2006)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. M. S. Patterson, B. Chance, and B. C. Wilson, "Time resolved reflectance and transmittance for the noninvasive measurement of tissue optical properties," Appl. Opt. 28, 2331-2336 (1989). [CrossRef] [PubMed]
  2. M. S. Patterson, J. D. Moulton, B. C. Wilson, K. W. Berndt, and J. R. Lakowicz, "Frequency-domain reflectance for the determination of the scattering and absorption properties of tissue," Appl. Opt. 30, 4474-4476 (1991). [CrossRef] [PubMed]
  3. R. A. J. Groenhuis, J. J. Tenbosch, and H. A. Ferwerda, "Scattering and absorption of turbid materials determined from reflection measurements. 2: Measuring method and calibration," Appl. Opt. 22, 2463-2467 (1983). [CrossRef] [PubMed]
  4. S. J. Matcher, M. Cope, and D. T. Delpy, "In vivo measurements of the wavelength dependence of tissue-scattering coefficients between 760 and 900 nm measured with time-resolved spectroscopy," Appl. Opt. 36, 386-396 (1997). [CrossRef] [PubMed]
  5. V. Ntziachristos and B. Chance, "Accuracy limits in the determination of absolute optical properties using time-resolved NIR spectroscopy," Med. Phys. 28, 1115-1124 (2001). [CrossRef] [PubMed]
  6. B. W. Pogue and M. S. Patterson, "Frequency-domain optical-absorption spectroscopy of finite tissue volumes using diffusion-theory," Phys. Med. Biol. 39, 1157-1180 (1994). [CrossRef] [PubMed]
  7. J. B. Fishkin, O. Coquoz, E. R. Anderson, M. Brenner, and B. J. Tromberg, "Frequency-domain photon migration measurements of normal and malignant tissue optical properties in a human subject," Appl. Opt. 36, 10-20 (1997). [CrossRef] [PubMed]
  8. M. G. Nichols, E. L. Hull, and T. H. Foster, "Design and testing of a white-light, steady-state diffuse reflectance spectrometer for determination of optical properties of highly scattering systems," Appl. Opt. 36, 93-104 (1997). [CrossRef] [PubMed]
  9. R. M. P. Doornbos, R. Lang, M. C. Aalders, F. W. Cross, and H. J. C. M. Sterenborg, "The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steady-state diffuse reflectance spectroscopy," Phys. Med. Biol. 44, 967-981 (1999). [CrossRef] [PubMed]
  10. J. S. Dam, C. B. Pedersen, T. Dalgaard, P. E. Fabricius, P. Aruna, and S. Andersson-Engels, "Fiber-optic probe for noninvasive real-time determination of tissue optical properties at multiple wavelengths," Appl. Opt. 40, 1155-1164 (2001). [CrossRef]
  11. L. H. Wang and S. L. Jacques, "Use of a laser beam with an oblique angle of incidence to measure the reduced scattering coefficient of a turbid medium," Appl. Opt. 34, 2362-2366 (1995). [CrossRef] [PubMed]
  12. A. Kienle, L. Lilge, M. S. Patterson, R. Hibst, R. Steiner, and B. C. Wilson, "Spatially resolved absolute diffuse reflectance measurements for noninvasive determination of the optical scattering and absorption coefficients of biological tissue," Appl. Opt. 35, 2304-2314 (1996). [CrossRef] [PubMed]
  13. T. H. Pham, F. Bevilacqua, T. Spott, J. S. Dam, B. J. Tromberg, and S. Andersson-Engels, "Quantifying the absorption and reduced scattering coefficients of tissuelike turbid media over a broad spectral range with noncontact Fourier-transform hyperspectral imaging," Appl. Opt. 39, 6487-6497 (2000). [CrossRef]
  14. T. J. Farrell, M. S. Patterson, and B. Wilson, "A diffusion-theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical-properties in vivo," Med. Phys. 19, 879-888 (1992). [CrossRef] [PubMed]
  15. L. H. Wang, S. L. Jacques, and L. Q. Zheng, "MCML--Monte-Carlo modeling of light transport in multilayered tissues," Comput. Methods Programs Biomed. 47, 131-146 (1995). [CrossRef] [PubMed]
  16. J. A. Raty and K. E. Peiponen, "Reflectance study of milk in the UV-visible range," Appl. Spectrosc. 53, 1123-1127 (1999). [CrossRef]
  17. R. Cubeddu, C. D'Andrea, A. Pifferi, P. Taroni, A. Torricelli, G. Valentini, M. Ruiz-Altisent, C. Valero, C. Ortiz, C. Dover, and D. Johnson, "Time-resolved reflectance spectroscopy applied to the nondestructive monitoring of the internal optical properties in apples," Appl. Spectrosc. 55, 1368-1374 (2001). [CrossRef]
  18. C. L. Crofcheck, F. A. Payne, and M. P. Menguc, "Characterization of milk properties with a radiative transfer model," Appl. Opt. 41, 2028-2037 (2002). [CrossRef] [PubMed]
  19. D. G. Fraser, R. B. Jordan, R. Kunnemeyer, and V. A. McGlone, "Light distribution inside mandarin fruit during internal quality assessment by NIR spectroscopy," Postharvest Biol. Technol. 27, 185-196 (2003). [CrossRef]
  20. C. Valero, M. Ruiz-Altisent, R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, G. Valentini, D. Johnson, and C. Dover, "Selection models for the internal quality of fruit, based on time domain laser reflectance spectroscopy," Biosyst. Eng. 88, 313-323 (2004). [CrossRef]
  21. R. Lu, "Imaging spectroscopy for assessing internal quality of apple fruit," in ASAE Annual International Meeting (American Society of Agricultural and Biological Engineers, 2003), paper 036012.
  22. R. Lu and Y. R. Chen, "Hyperspectral imaging for safety inspection of food and agricultural products," in Pathogen Detection and Remediation for Safe Eating, Y.-R. Chen, ed., Proc. SPIE 3544, 121-133 (1998).
  23. H. J. van Staveren, C. J. M. Moes, J. van Marle, S. A. Prahl, and M. J. C. van Gemert, "Light scattering in Intralipid-10% in the wavelength range of 400-1100 nm," Appl. Opt. 30, 4507-4514 (1991). [CrossRef] [PubMed]
  24. R. A. J. Groenhuis, H. A. Ferwerda, and J. J. Tenbosch, "Scattering and absorption of turbid materials determined from reflection measurements. 1: Theory," Appl. Opt. 22, 2456-2462 (1983). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited