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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: James C. Wyant
  • Vol. 45, Iss. 33 — Nov. 20, 2006
  • pp: 8560–8565

Position of the prism in a dispersion-compensated acousto-optic deflector for multiphoton imaging

Kun Bi, Shaoqun Zeng, Songchao Xue, Jian Sun, Xiaohua Lv, Derong Li, and Qingming Luo  »View Author Affiliations


Applied Optics, Vol. 45, Issue 33, pp. 8560-8565 (2006)
http://dx.doi.org/10.1364/AO.45.008560


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Abstract

The performance of a dispersion-compensated acousto-optic deflector (AOD) for steering femtosecond laser pulses was examined with the prism located before or after the AOD, which is regarded as prism-AOD and AOD-prism, respectively. Comparisons are made over parameters including the spot spatial pattern, output pulse width, scanning linearity, the field of view, and the transmission rate. Fluorescence images of 170   nm diameter beads and cells were measured to provide an overall evaluation for these femtosecond laser beam scanning configurations. On the basis of these experiments, the prism-AOD configuration is concluded to be more advantageous for the purpose of simultaneous compensation for the spatial and temporal dispersion.

© 2006 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.5810) Medical optics and biotechnology : Scanning microscopy

History
Original Manuscript: April 21, 2006
Revised Manuscript: July 20, 2006
Manuscript Accepted: August 8, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Kun Bi, Shaoqun Zeng, Songchao Xue, Jian Sun, Xiaohua Lv, Derong Li, and Qingming Luo, "Position of the prism in a dispersion-compensated acousto-optic deflector for multiphoton imaging," Appl. Opt. 45, 8560-8565 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-33-8560


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References

  1. W. R. Zipfel, R. W. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the biosciences," Nat. Biotechnol. 21, 1368-1376 (2003). [CrossRef]
  2. D. R. Larson, W. R. Zipfel, R. M. Williams, S. W. Clark, M. P. Bruchez, F. W. Wise, and W. W. Webb, "Water-soluble quantum dots for multiphoton fluorescence imaging in vivo," Science 300, 1434-1436 (2003). [CrossRef] [PubMed]
  3. W. Denk, D. W. Piston, and W. W. Webb, "Two-photon molecular excitation in laser-scanning microscopy," in Handbook of Biological Confocal Microscopy, J.B. Pawley, ed. (Plenum, 1995), pp. 445-458.
  4. S. R. Goldstein, T. Hubin, S. Rosenthal, and C. Washburn, "A confocal video-rate laser-beam scanning reflected-light microscopy with no moving parts," J. Microsc. 157, 29-38 (1990). [CrossRef] [PubMed]
  5. A. Bullen, S. S. Patel, and P. Saggau, "High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators," Biophys. J. 73, 477-491 (1997). [CrossRef] [PubMed]
  6. V. Iyer, B. E. Losavio, and P. Saggau, "Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy," J. Biomed. Opt. 8, 460-471 (2003). [CrossRef] [PubMed]
  7. J. D. Lechleiter, D. T. Lin, and I. Sieneart, "Multi-photon laser scanning microscopy using an acoustic optical deflector," Biophys. J. 83, 2292-2299 (2002). [CrossRef] [PubMed]
  8. R. D. Roorda, T. M. Hohl, R. Toledo-Crow, and G. Miesenbock, "Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes," J. Neurophysiol. 92, 609-621 (2004). [CrossRef] [PubMed]
  9. S. Zeng, X. Lv, C. Zhan, W. R. Chen, W. Xiong, S. L. Jacques, and Q. Luo, "Simultaneous compensation of spatial and temporal dispersion of acousto-optical deflectors for two-dimensional scanning with a single prism," Opt. Lett. 31, 1091-1093 (2006). [CrossRef] [PubMed]
  10. M. Nakazawa, T. Nakashima, and H. Kubota, "Optical pulse compression using a TeO2 acousto-optical light deflector," Opt. Lett. 13, 120-122 (1988). [CrossRef] [PubMed]

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