Optical sectioning by optical scanning holography and a Wiener filter
Applied Optics, Vol. 45, Issue 5, pp. 872-879 (2006)
http://dx.doi.org/10.1364/AO.45.000872
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Abstract
I propose a novel digital technique that reduces defocus noise in the reconstruction of the sectional images from the complex hologram of a thick object. In three-dimensional microscopy applications of holography, reducing the defocused light scattered from outside the focused plane is an important issue. In this technique I first extract a complex hologram of a thick object by using optical scanning holography. After that, I separate the power spectra of the focused and defocused planes from the complex hologram. Finally, I construct a Wiener filter by use of the power spectra. The Wiener filter reduces the defocus noise in the reconstruction of the sectional image of the focused plane. Computer simulations show that the proposed Wiener filter reduces the defocus noise and provides the sectional images.
© 2006 Optical Society of America
OCIS Codes
(070.2580) Fourier optics and signal processing : Paraxial wave optics
(090.1760) Holography : Computer holography
(100.3010) Image processing : Image reconstruction techniques
(180.6900) Microscopy : Three-dimensional microscopy
ToC Category:
Digital/Electronic Holographic Microscopy
History
Original Manuscript: February 28, 2005
Revised Manuscript: April 26, 2005
Manuscript Accepted: April 28, 2005
Virtual Issues
Vol. 1, Iss. 3 Virtual Journal for Biomedical Optics
Citation
Taegeun Kim, "Optical sectioning by optical scanning holography and a Wiener filter," Appl. Opt. 45, 872-879 (2006)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-45-5-872
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